Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01436-0012-0000-9
Chemical Name:	3-Chloromethylpyridine hydrochloride
CASRN:	6959-48-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	579799
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	129	82	40	47
			65	101	81	42
			68	97	108	53
			50	73	78	52
Test Chemical	3-Chloromethylpyridine hydrochloride	6.25	20	24	44	73	84*
			24	28	68	95	84*
		12.5	55	54	163	99
			61r	0	110	60
		25	37#	36	100	91	122*
			28	24	129	153	122*
		50	35	10	307	290	341*
			20	8	237	392	341*
		100

Positive Control	Ethyl Methane Sulfonate	200	65	74	313	160	166*
Positive Control			67	69	345	171	166*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	90	95	147	54	61
			91	103	179	66
			89	99	189	71
			112	103	173	52
Test Chemical	3-Chloromethylpyridine hydrochloride	2.5	62	86	168	90	79
			75	102	154	68	79
		5	67	65	210	104	110*
			67	72	233	116	110*
		10	51	56	235	154	117*
			64	62	154	80	117*
		20	44	38	212	162	153*
			60	48	257	143	153*
		40	38	4	528	469	422*
			51	5	571	376	422*
		80

Positive Control	Ethyl Methane Sulfonate	250	58	49	851	493	428*
Positive Control			64	58	699	362	428*

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	78	106	134	57	59
			73	110	119	54
			58#	97	105	60
			64	87	120	63
Test Chemical	3-Chloromethylpyridine hydrochloride	6.25	77	95	151	65	54
			85#	116	107	42	54
		12.5	78#	126	153	66	61
			61	66	104	57	61
		25	45	62	122	90	81
			59	90	128	73	81
		50	67	62	207	103	91
			70	65	166	79	91
		100

Positive Control	3-Methylcholanthrene	2.5	57	83	173	101	83
Positive Control			86	148	166	65	83

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	70	85	81	39	47
			78	112	115	49
			85	100	107	42
			76	102	133	59
Test Chemical	3-Chloromethylpyridine hydrochloride	20	79	71	240	101	90*
			83	82	196	79	90*
		40	55	42	340	205	206*
			50	48	311	208	206*
		60	48	5	670	464	408*
			43	6	448	351	408*
		80

Positive Control	3-Methylcholanthrene	2.5	35	8	421	403	395*
Positive Control			36#	7	420	387	395*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination