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Study Information
Study Attribute Value
CEBS Accession Number: 002-01436-0013-0000-0
Chemical Name: 3-Chloromethylpyridine hydrochloride
CASRN: 6959-48-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G05039
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 80 105 52 22 34
    104 113 111 36
    71 84 112 53
    84 97 65 26
Test Chemical 3-Chloromethylpyridine hydrochloride 
7.8       
 99 114 125 42 40
    93 115 105 38
  
15.6       
 78 91 111 48 52
    70 89 120 57
  
31.3       
 89 83 187 70 77*
    80 74 202 84
  
46.9       
 76 45 313 138 132*
    72 48 273 127
  
62.5       
 54 7 411 252 248*
    46 6 334 243
  
93.8       
   
Positive Control Ethyl Methane Sulfonate 
500         
 55 34 929 566 597*
    54 32 1010 627

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 74 100 81 37 36
    52 116 58 37
    55 84 57 35
    45r 90 45 33
Test Chemical 3-Chloromethylpyridine hydrochloride 
15.6       
 65 88 116 60 55
    69 107 104 50
  
25         
 60# 79 256 143 121*
    60 66 177 98
  
31.3       
 61# 87 230 127 129*
    66# 65 261 132
  
50         
 69 9 564 274 313*
    39 4 406 352
  
62.5       
 50 5 387 259
   
  
75         
   
Positive Control Ethyl Methane Sulfonate 
500         
 16# 17 581 1230 1008*
    23 22 542 786

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 66 88 39 20 18
    54 96 26 16
    60 115 29 16
    68 101 43 21
Test Chemical 3-Chloromethylpyridine hydrochloride 
10         
 61 124 47 26 21
    69 125 34 16
  
20         
 53 92 56 35 42*
    58 115 85 49
  
30         
 51 97 64 42 44*
    46 81 64 46
  
40         
 49 83 89 61 59*
    55 83 94 57
  
50         
 66 76 140 71 81*
    51 54 141 92
  
60         
 46 19 165 120 100*
    58 30 137 79
Positive Control Ethyl Methane Sulfonate 
500         
 19 13 643 1159 1079*
    19 15 625 1096
    20 13 594 982

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 91 89 94 34 33
    105 124 79 25
    98 85 99 34
    109 102 120 37
Test Chemical 3-Chloromethylpyridine hydrochloride 
3.9       
 114 57 124 36
    121r 73 158 44
  
7.8       
 93 35 145 52 50
    94 33 135 48
  
15.6       
 99 26 350 118 91*
    96 30 185 64
  
31.3       
 94 23 294 104 97*
    83 24 222 90
  
62.5       
 81 8 442 181 195*
    76 7 479 210
  
93.8       
   
Positive Control 3-Methylcholanthrene 
5         
 48 12 668 469 427*
    52 24 600 385

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 102 100 144 47 38
    93# 128 84 30
    94 60 105 37
    92 112 102 37
Test Chemical 3-Chloromethylpyridine hydrochloride 
3.13      
 85 76 113 44 48
    80 89 124 52
  
6.25      
 94# 83 88.5 31 41
    62 61 94 51
  
12.5       
 92 36 144 52 56
    80 42 141 59
  
25         
 90 50 166 61 61*
    87 39 159 61
  
50         
 83 39 236 94 90*
    94 33 238 85
  
100         
   
Positive Control 3-Methylcholanthrene 
5         
 94 56 549 195 215*
    76 48 538 235

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 91 112 120 44 48
    90 78 145 54
    77 107 110 47
    85 103 120 47
Test Chemical 3-Chloromethylpyridine hydrochloride 
10         
 51 62 68 45 43
    81 61 99 41
  
20         
 54 36 164 101 93*
    64 37 164 86
  
30         
 54 29 237 148 121*
    81 39 227 94
  
40         
 66 27 299 152 160*
    55 21 278 167
  
50         
 50 14 308 204 161*
    74 33 261 118
  
60         
 75 26 257 114 124*
    83 23 330 133
Positive Control 3-Methylcholanthrene 
5         
 42 8 354 284 267*
    30 6 222 249

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination