Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01452-0008-0000-2
Chemical Name:	3-Nitropropionic acid
CASRN:	504-88-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G46070
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	60	90	108	60	48
			72	120	68	32
			65	104	96	50
			51	86	80	52
Test Chemical	3-Nitropropionic acid	26	68	45	157	77	67
			80	64	137	57	67
		51	68	33	284	140	152*
			58	36	286	164	152*
		102	61	36	363	198	203*
			62	30	386	209	203*
		205	49	23	313	211	214*
			43	27	282	217	214*
		410	51	21	376	245	213*
			46	21	252	181	213*
		512	52	20	369	239	212*
			48	18	265	186	212*
		640	41	14	360	294	278*
			40	11	313	263	278*
		800	34	7	539	531	495*
			30	7	411	459	495*
		1250

Positive Control	Ethyl Methane Sulfonate	500	44	43	1008	772	756*
			47	44	1035	734
			46	44	1049	763

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	66	103	80	41	40
			72	101	80	37
			69	96	83	40
			70	100	92	44
Test Chemical	3-Nitropropionic acid	210	45	24	379	284
		262	51	26	326	212	210*
			51	26	319	209	210*
		328	51#	23	349.5	231	215*
			56	24	333	199	215*
		410	62	35	432	232	223*
			60	33	386	214	223*
		512	54	33	353	217	200*
			70	37	380	182	200*
		640	58	34	303	176	169*
			68	40	333	163	169*
		800	64#	37	317	164
		1000	58	32	341	197	198*
			61	31	363	198	198*
Positive Control	Ethyl Methane Sulfonate	500	33	27	871	889	822*
			35	30	893	842
			38	36	827	735

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	72	98	106	49	42
			74	95	117	53
			68	95	81	40
			76	112	65	28
Test Chemical	3-Nitropropionic acid	328	74	83	161	72	81*
			62	60	168	90	81*
		640	68	53	183	89
		800	63	40	251	132	129*
			55	36	205	125	129*
		1000	41	18	465	377	289*
			48	19	289	201	289*
Positive Control	3-Methylcholanthrene	5	45	29	574	424	391*
			44	28	517	393
			45	31	474	355

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	73	104	62	29	31
			65	86	79	41
			57	91	37	22
			77	119	73	32
Test Chemical	3-Nitropropionic acid	512	77	95	114	50	66*
			67	79	165	82	66*
		640	66	70	177	90	83*
			77	84	177	77	83*
		800	52	45	161	104	88*
			78	62	167	72	88*
		1000	58	38	187	108	107*
			53	41	167	105	107*
		1250	6r	1	109	661
			16	4	164	342
Positive Control	3-Methylcholanthrene	5	56	63	363	217	227*
			54	58	352	217
			52	50	385	247

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination