Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01452-0009-0000-3
Chemical Name:	3-Nitropropionic acid
CASRN:	504-88-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G63977
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	74	99	44	20	26
			90	90	84	31
			79#	102	68	29
			75	109	52	23
Test Chemical	3-Nitropropionic acid	62.5	77	69	86	37	32
			82	72	66	27	32
		125	79	49	161	68	68*
			82	47	166	67	68*
		250	69	44	139	67	67*
			79	46	159	68	67*
		500	66	47	78	40	37
			76	62	79	34	37
		750	96	60	99	34	40
			82	48	111	45	40
		1000

Positive Control	Ethyl Methane Sulfonate	500	42	23	990	786	804*
Positive Control			48#	26	1185	823	804*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	87	87	32	12	15
			74	116	44	20
			73	90	31	14
			87	107	39	15
Test Chemical	3-Nitropropionic acid	200	89	61	88	33	41*
			96#	72	104	36
			74	60	122	55
		300	66#	77	55.5	28	30*
			89	60	98	37
			97	83	74	26
		400	78	59	93	40	31*
			73	83	50	23
			84	67	75	30
		600	79	63	110	46	50*
			67	26	138	69
			84	74	89	35
		800


Positive Control	Ethyl Methane Sulfonate	500	40	26	610	508	527*
			39	27	569	488
			37	25	643	585

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	86	73	41	16	19
			94	123	54	19
			93	99	71	25
			94	106	49	17
Test Chemical	3-Nitropropionic acid	100	99	54	108	36	35*
			96	44	114	40
			87	63	75	29
		200	104	41	148	48	55*
			94	43	162	58
			90	38	159	59
		400	82	37	151	62	52*
			87	41	127	49
			92	51	123	45
		600	83	64	92	37	31*
			102	67	77	25
			93	64	90	32
		700	99	66	91	31	33*
			93	71	80	29
			85	71	99	39
		800	92	55	97	35	34*
			101	55	102	34
			95	63	91	32
		1000


Positive Control	Ethyl Methane Sulfonate	500	77	52	835	361	533*
			48	23	1077	748
			56	35	825	491

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	80	80	57	24	26
			92	109	76	28
			98	99	83	28
			90	112	66	25
Test Chemical	3-Nitropropionic acid	250	93	54	98	35	37
			71	48	81	38	37
		500	81	53	91	38	37
			84	51	90	36	37
		750	68	40	151	74	61*
			73	60	107	49	61*
		1000	50	13	293	197
			46r	13	0	0
		1200

Positive Control	3-Methylcholanthrene	5	43	26	304	236	250*
			50	24	369	246
			43#	23	345	269

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	102	92	88	29	26
			104	98	85	27
			81	101	44	18
			101	108	89	29
Test Chemical	3-Nitropropionic acid	125	87	54	83	32	37
			87	48	86	33
			95	50	129	45
		250	83	44	115	46	46*
			88	45	118	45
			94	46	136	48
		500	77	46	138	60	60*
			77	44	140	60
			93	51	169	61
		750	67	22	373	187	163*
			76	19	378	166
			53	23	218	137
Positive Control	3-Methylcholanthrene	5	44	19	426	320	322*
			33	16	333	333
			43	16	406	314

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	66	68	131	66	50
			96	124	121	42
			93	102	127	45
			86	106	125	48
Test Chemical	3-Nitropropionic acid	200	72	37	262	121	134*
			90	39	355	132
			75	32	340	150
		400	79	37	417	176	153*
			85	40	302	118
			79	29	389	164
		500	95	52	338	119	168*
			65	31	385	198
			85	27	474	185
		600	71	28	374	176	174*
			85	32	434	171
			71	34	370	175
		700	49	13	377	258	263*
			53	11	441	276
			54	16	409	255
		800	19	1	366	654	580*
			26	2	397	506
			17r	1	412	824
		1000


Positive Control	3-Methylcholanthrene	5	51	24	461	300	338*
			41	19	434	353
			45	15	482	361

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination