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Study Information
Study Attribute Value
CEBS Accession Number: 002-01456-0010-0000-9
Chemical Name: 3-Sulfolene
CASRN: 77-79-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 964398
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 63 91 132 70 61
    75 91 118 52
    71 103 121 57
    75 116 149 66
Test Chemical 3-Sulfolene 
312.5       
 57 96 130 76 75
    58 93 129 75
  
625         
 59 91 149 84 72
    72 94 129 60
  
1250         
 73 96 133 61 59
    66 96 113 57
  
2500         
 65 91 127 65 59
    70 99 112 53
  
5000         
 68 83 102 50 52
    76 83 124 55
Positive Control Methyl Methane Sulfonate 
20         
 29 21 311 357 399*
    28 17 363 440
Methyl Methane Sulfonate 
250         
 58 87 554 320 304*
    61 85 528 287

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 81 108 110 46 54
    72 97 123 57
    71 94 112 53
    76 100 142 62
Test Chemical 3-Sulfolene 
1000         
 75 108 100 45 53
    78 128 141 61
  
2000         
 58 100 107 61 56
    69 119 106 51
  
3000         
 48 68 93 65 52
    62 102 72 39
  
4000         
 43 81 110 85 75
    61 84 118 64
  
5000         
 65 107 165 84 71
    58 102 101 59
Positive Control Methyl Methane Sulfonate 
15         
 24 20 314 430 390*
    35 25 363 351

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 95 215 87 76
    77 112 169 73
    79 100 153 65
    83 94 197 79
Test Chemical 3-Sulfolene 
1000         
 85 107 195 76 77
    87 95 202 77
  
2000         
 93 111 197 70 73
    87 104 199 76
  
3000         
 90 82 174 65 64
    106 95 201 63
  
4000         
 87 107 164 63 65
    86 98 173 67
  
5000         
 89 84 166 62 66
    87 93 182 70
Positive Control 3-Methylcholanthrene 
2.5       
 32 10 353 372 433*
    26 8 381 495

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 55 80 74 45 41
    67 96 86 43
    77 109 78 34
    93 114 115 41
Test Chemical 3-Sulfolene 
1000         
 50 62 59 39 51
    44 72 82 63
  
2000         
 61 100 88 48 50
    60 111 93 52
  
3000         
 61 106 103 57 54
    54 87 81 50
  
4000         
 79 88 125 53 51
    70 92 104 50
  
5000         
 78 107 107 46 53
    72 96 131 61
Positive Control 3-Methylcholanthrene 
2.5       
 43 27 489 378 411*
    32 31 432 445

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination