Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01456-0010-0000-9
Chemical Name:	3-Sulfolene
CASRN:	77-79-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	964398
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	91	132	70	61
			75	91	118	52
			71	103	121	57
			75	116	149	66
Test Chemical	3-Sulfolene	312.5	57	96	130	76	75
			58	93	129	75	75
		625	59	91	149	84	72
			72	94	129	60	72
		1250	73	96	133	61	59
			66	96	113	57	59
		2500	65	91	127	65	59
			70	99	112	53	59
		5000	68	83	102	50	52
			76	83	124	55	52
Positive Control	Methyl Methane Sulfonate	20	29	21	311	357	399*
			28	17	363	440	399*
	Methyl Methane Sulfonate	250	58	87	554	320	304*
			61	85	528	287	304*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	108	110	46	54
			72	97	123	57
			71	94	112	53
			76	100	142	62
Test Chemical	3-Sulfolene	1000	75	108	100	45	53
			78	128	141	61	53
		2000	58	100	107	61	56
			69	119	106	51	56
		3000	48	68	93	65	52
			62	102	72	39	52
		4000	43	81	110	85	75
			61	84	118	64	75
		5000	65	107	165	84	71
			58	102	101	59	71
Positive Control	Methyl Methane Sulfonate	15	24	20	314	430	390*
Positive Control			35	25	363	351	390*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	95	215	87	76
			77	112	169	73
			79	100	153	65
			83	94	197	79
Test Chemical	3-Sulfolene	1000	85	107	195	76	77
			87	95	202	77	77
		2000	93	111	197	70	73
			87	104	199	76	73
		3000	90	82	174	65	64
			106	95	201	63	64
		4000	87	107	164	63	65
			86	98	173	67	65
		5000	89	84	166	62	66
			87	93	182	70	66
Positive Control	3-Methylcholanthrene	2.5	32	10	353	372	433*
Positive Control			26	8	381	495	433*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	55	80	74	45	41
			67	96	86	43
			77	109	78	34
			93	114	115	41
Test Chemical	3-Sulfolene	1000	50	62	59	39	51
			44	72	82	63	51
		2000	61	100	88	48	50
			60	111	93	52	50
		3000	61	106	103	57	54
			54	87	81	50	54
		4000	79	88	125	53	51
			70	92	104	50	51
		5000	78	107	107	46	53
			72	96	131	61	53
Positive Control	3-Methylcholanthrene	2.5	43	27	489	378	411*
Positive Control			32	31	432	445	411*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination