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Study Information
Study Attribute Value
CEBS Accession Number: 002-01462-0002-0000-7
Chemical Name: 4,4'-Dimethoxydiphenylamine
CASRN: 101-70-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 918738_CA
Study Result: Weakly Positive

Study Result: Weakly Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Questionable 
No Activation  2  Weakly Positive 
Induced Rat Liver S9  2_S9  Questionable 
No Activation  3  Questionable 
Induced Rat Liver S9  3_S9  Test Failure 

Trial #:1   Activation: No Activation    Date: 03/13/1986    Harvest Time: 10 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 7 0.035 3.0 1 0.005 0.5 6 0.030 2.5 0 0.000 0.0
Test Chemical: 4,4'-Dimethoxydiphenylamine 
159         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
530         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
1590         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 50 52 1.040 44.0 8 0.160 16.0 24 0.480 28.0 20 0.400 2.0
Mitomycin-C 
5         
 50 64 1.280 56.0 17 0.340 30.0 27 0.540 32.0 20 0.400 2.0
Trend: -2.884 -1.346 -2.562  
Probability: 0.998 0.911 0.995  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/04/1986    Harvest Time: 12 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 10 0.050 4.0 3 0.015 1.5 7 0.035 3.0 0 0.000 0.0
Test Chemical: 4,4'-Dimethoxydiphenylamine 
132         
 200 44 0.220 11.5 16 0.080 7.5 18 0.090 6.5 10 0.050 1.0 
440         
 200 36 0.180 8.5 9 0.045 4.5 17 0.085 5.0 10 0.050 1.0 
1320         
 200 11 0.055 5.5 5 0.025 2.5 6 0.030 3.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 34 0.680 40.0 8 0.160 16.0 16 0.320 26.0 10 0.200 1.0
Trend: 0.193 0.007 -0.222  
Probability: 0.423 0.497 0.588  


Trial #:2   Activation: No Activation    Date: 01/21/1988    Harvest Time: 20 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 2 0.010 1.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: 4,4'-Dimethoxydiphenylamine 
504         
 200 15 0.075 4.5 3 0.015 1.5 12 0.060 3.0 0 0.000 0.0 
1501         
 200 17 0.085 4.5 9 0.045 2.0 8 0.040 3.5 0 0.000 0.0 
2498         
 200 17 0.085 7.5 6 0.030 2.5 11 0.055 5.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.2       
 200 52 0.260 22.0 21 0.105 10.5 29 0.145 13.5 2 0.010 2.0
Mitomycin-C 
5         
 50 287 5.740 98.0 49 0.980 56.0 45 0.900 50.0 193 3.860 22.0
Trend: 2.627 1.196 2.547  
Probability: 0.004 0.116 0.005  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 04/22/1986    Harvest Time: 12 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 14 0.070 4.0 5 0.025 1.5 9 0.045 3.5 0 0.000 0.0
Test Chemical: 4,4'-Dimethoxydiphenylamine 
264         
 200 28 0.140 10.0 10 0.050 4.0 18 0.090 7.0 0 0.000 0.0 
530         
 200 19 0.095 5.5 5 0.025 2.0 13 0.065 4.0 1 0.005 1.0 
795         
 200 26 0.130 8.0 11 0.055 3.5 14 0.070 6.0 1 0.005 1.0 
1060         
 200 15 0.075 7.5 5 0.025 2.5 10 0.050 5.0 0 0.000 0.0 
1590         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 24 0.480 28.0 12 0.240 20.0 12 0.240 16.0 0 0.000 0.0
Trend: 0.762 0.374 0.339  
Probability: 0.223 0.354 0.367  


Trial #:3   Activation: No Activation    Date: 06/24/1986    Harvest Time: 20.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4,4'-Dimethoxydiphenylamine 
560         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
1130         
 200 4 0.020 0.5 3 0.015 0.5 1 0.005 0.5 0 0.000 0.0 
1690         
 300 45 0.150 2.7 3 0.010 0.7 2 0.007 0.7 40 0.133 4.0
Positive Control: Mitomycin-C 
 0.2       
 200 106 0.530 33.0 62 0.310 25.5 33 0.165 12.0 11 0.055 2.0
Mitomycin-C 
5         
 10 85 8.500 100.0 2 0.200 10.0 2 0.200 10.0 81 8.100 9.0
Trend: 2.722 1.555 1.047  
Probability: 0.003 0.060 0.148  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 01/27/1988    Harvest Time: 12 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 18 0.090 7.5 3 0.015 1.5 10 0.050 4.0 5 0.025 5.0
Test Chemical: 4,4'-Dimethoxydiphenylamine 
128         
 200 32 0.160 13.0 8 0.040 4.0 21 0.105 9.5 3 0.015 3.0 
253         
 200 26 0.130 10.5 11 0.055 5.5 11 0.055 4.5 4 0.020 4.0 
505         
 200 27 0.135 10.5 3 0.015 1.5 21 0.105 9.0 3 0.015 3.0 
760         
 200 47 0.235 17.0 12 0.060 4.5 27 0.135 11.5 8 0.040 8.0 
1010         
 200 29 0.145 12.0 9 0.045 4.5 17 0.085 6.5 3 0.015 3.0
Positive Control: Cyclophosphamide 
50         
 50 37 0.740 42.0 10 0.200 18.0 12 0.240 22.0 15 0.300 6.0
Trend: 1.825 1.015 1.617  
Probability: 0.034 0.155 0.053