Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-01465-0016-0000-5
Chemical Name: 4,4'-Methylenebis(2-chloroaniline)
CASRN: 101-14-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G87947
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 98 93 30 34
    108 102 121 38
Test Chemical 4,4'-Methylenebis(2-chloroaniline) 
51.7       
 112 64 165 49 49
    95 55 138 48
  
63.8       
 111 75 173 52 48
    110 68 143 43
  
78.7       
 104 49 149 48 49
    98 33 151 51
  
87.5       
 95 28 129 45 46
    95 24 131 46
  
97.2       
 85 12 81 32 40
    91 12 131 48
  
108         
   
Positive Control Ethyl Methane Sulfonate 
500         
 63 35 963 508 502*
    62 37 919 497

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 106 92 31 31
    90 94 84 31
Test Chemical 4,4'-Methylenebis(2-chloroaniline) 
41.8       
 84 78 78 31 37
    86 77 113 44
  
51.6       
 93 77 99 35 43
    84 69 129 51
  
63.7       
 90 52 133 49 43
    103 70 114 37
  
78.7       
 79 28 104 44 48
    85 30 130 51
  
87.5       
 85 17 97 38
  
97.2       
    56 3 146 87
  
108         
   
Positive Control Ethyl Methane Sulfonate 
500         
 55 34 1003 612 656*
    48 34 1008 700

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 111 105 48 14 18
    106 95 67 21
Test Chemical 4,4'-Methylenebis(2-chloroaniline) 
5.4       
 99 99 64 22 22
    107 105 72 23
  
9.1       
 107 96 49 15 20
    104 95 78 25
  
15.1       
 92 73 80 29 26
    102 85 71 23
  
25.2       
 103 78 69 22 25
    104 72 90 29
  
42         
 90 48 118 44 39*
    81 43 81 33
  
70         
 62 23 122 66 67*
    75 27 151 67
Positive Control 3-Methylcholanthrene 
6         
 38 17 404 356 278*
    59 26 352 199

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 101 232 86 92
    94 99 274 97
Test Chemical 4,4'-Methylenebis(2-chloroaniline) 
3.3       
 96 106 259 90 103
    84 95 295 117
  
5.4       
 91 85 312 114 117
    83 89 298 120
  
9.1       
 81 80 284 117 115
    98 85 329 112
  
15.1       
 90 59 367 135 135
    79 55 319 135
  
25.2       
 62 22 384 205 195*
    72 24 399 185
  
42         
 46 5 437 317 324*
    42 5 418 332
  
70         
   
Positive Control 3-Methylcholanthrene 
5         
 47 23 492 351 357*
    44 23 473 362

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination