Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01465-0016-0000-5
Chemical Name:	4,4'-Methylenebis(2-chloroaniline)
CASRN:	101-14-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G87947
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	98	93	30	34
Vehicle Control			108	102	121	38	34
Test Chemical	4,4'-Methylenebis(2-chloroaniline)	51.7	112	64	165	49	49
			95	55	138	48	49
		63.8	111	75	173	52	48
			110	68	143	43	48
		78.7	104	49	149	48	49
			98	33	151	51	49
		87.5	95	28	129	45	46
			95	24	131	46	46
		97.2	85	12	81	32	40
			91	12	131	48	40
		108

Positive Control	Ethyl Methane Sulfonate	500	63	35	963	508	502*
Positive Control			62	37	919	497	502*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	106	92	31	31
Vehicle Control			90	94	84	31	31
Test Chemical	4,4'-Methylenebis(2-chloroaniline)	41.8	84	78	78	31	37
			86	77	113	44	37
		51.6	93	77	99	35	43
			84	69	129	51	43
		63.7	90	52	133	49	43
			103	70	114	37	43
		78.7	79	28	104	44	48
			85	30	130	51	48
		87.5	85	17	97	38
		97.2
			56	3	146	87
		108

Positive Control	Ethyl Methane Sulfonate	500	55	34	1003	612	656*
Positive Control			48	34	1008	700	656*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	111	105	48	14	18
Vehicle Control			106	95	67	21	18
Test Chemical	4,4'-Methylenebis(2-chloroaniline)	5.4	99	99	64	22	22
			107	105	72	23	22
		9.1	107	96	49	15	20
			104	95	78	25	20
		15.1	92	73	80	29	26
			102	85	71	23	26
		25.2	103	78	69	22	25
			104	72	90	29	25
		42	90	48	118	44	39*
			81	43	81	33	39*
		70	62	23	122	66	67*
			75	27	151	67	67*
Positive Control	3-Methylcholanthrene	6	38	17	404	356	278*
Positive Control			59	26	352	199	278*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	101	232	86	92
Vehicle Control			94	99	274	97	92
Test Chemical	4,4'-Methylenebis(2-chloroaniline)	3.3	96	106	259	90	103
			84	95	295	117	103
		5.4	91	85	312	114	117
			83	89	298	120	117
		9.1	81	80	284	117	115
			98	85	329	112	115
		15.1	90	59	367	135	135
			79	55	319	135	135
		25.2	62	22	384	205	195*
			72	24	399	185	195*
		42	46	5	437	317	324*
			42	5	418	332	324*
		70

Positive Control	3-Methylcholanthrene	5	47	23	492	351	357*
Positive Control			44	23	473	362	357*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination