Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01466-0008-0000-7
Chemical Name:	4,4'-Methylenebis(N,N-dimethyl)benzenamine
CASRN:	101-61-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G03676
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	54	107	64	39	44
			72	94	102	48
			54	100	67	41
			62	99	90	49
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	7.8	64	92	101	53	51
			70	113	104	49	51
		15.6	54	82	84	52	46
			57	102	67	39	46
		31.3	69	80	124	60	50
			70	88	83	40	50
		62.5	55	64	73	44	51
			73	88	126	58	51
		125

Positive Control	Ethyl Methane Sulfonate	500	36	26	608	563	622*
Positive Control			40	30	814	681	622*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	82	68	29	25
			85	124	59	23
			89	102	70	26
			77	92	52	23
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	30	81	101	60	25	28
			67	70	64	32	28
		60	95	98	74	26	34
			90	97	116	43	34
		80	87	69	75	29	31
			87	81	84	32	31
		100	79	56	65	27	29
			96	52	90	31	29
		120	65	18	98	50	52*
			49#	5	78	53	52*
Positive Control	Ethyl Methane Sulfonate	500	45	34	870	640	567*
Positive Control			55#	37	814.5	494	567*

Nonactivation Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	89	66	33	27
			97	123	67	23
			74	86	63	29
			72	102	54	25
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	15	103	100	75	24	29
			78	88	45	19
			83	93	107	43
		30	78	86	64	28	31
			73	78	100	46
			89	93	54	20
		60	105	75	120	38	30
			80	66	70	29
			97	72	67	23
		80	65	35	50	26	29
			74	25	68	31
			71	18	64	30
		100

			45	8	73	54
		120


Positive Control	Ethyl Methane Sulfonate	500	34	23	544	539	597*
			33	19	713	717
			36	26	584	536

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	90	112	48	53
			96	114	160	56
			112	102	160	48
			80	94	146	61
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	1.25	101	102	141	47	53
			84	105	148	59	53
		2.5	99	100	148	50	52
			95	106	157	55	52
		5	85	58	203	80	81
			74	40	184	83	81
		10	103	43	301	97	120*
			57	30	243	142	120*
		15	65	27	250	129	143*
			79	25	371	157	143*
		20	58	13	384	219	236*
			69	10	524	253	236*
		30	56	10	406	240

		40

Positive Control	3-Methylcholanthrene	5	38	16	412	360	360*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	79	81	27	24
			112	99	69	21
			105	100	92	29
			114	122	69	20
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	1.25	84	85	144	57
			121r	105	134	37
		2.5	90	86	146	54	47*
			108	102	131	40	47*
		5	75	74	159	71	61*
			98	67	152	52	61*
		10	71	24	172	81	88*
			81	24	231	95	88*
		15	85	25	206	80	83*
			82	18	209	85	83*
		20	39	5	148	128	127*
			54	14	174	107
			58	10	255	146
		30


Positive Control	3-Methylcholanthrene	5	40	11	498	420	453*
			46	10	639	466
			38	8	531	472

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination