Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01466-0009-0000-8
Chemical Name:	4,4'-Methylenebis(N,N-dimethyl)benzenamine
CASRN:	101-61-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G72954
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	100	113	51	46
			89	113	117	44
			83	101	126	51
			79	87	97	41
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	32.8	92	130	224	81	81*
			99	119	240	81	81*
		51.2	87	102	294	112	94*
			83	91	191	76	94*
		64	76	66	210	93	72
			106	86	162	51	72
		80	83	67	238	96	184*
			102	118	832	271	184*
		100	86	30	1292	502	562*
			69	26	1286	623	562*
Positive Control	Ethyl Methane Sulfonate	500	43	32	1008	778	761*
Positive Control			43	36	958	743	761*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	40r	87	135	113	79
			51	108	81	53
			56	104	153	92
			52	88	143	92
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	51	56	110	209	125	112
			50	82	147	98	112
		60	41	77	195	160	147*
			55	90	218	133	147*
		71	58	86	266	154	152*
			65	81	290	149	152*
		83	57	60	376	221	228*
			44	48	311	234	228*
		98	42	21	698	552	539*
			41	20	644	526	539*
		115	26	8	994	1283	1540*
			23	5	1213	1797	1540*
Positive Control	Ethyl Methane Sulfonate	500	35	53	751	712	700*
			37	46	735	668
			33	43	703	721

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77#	94	136.5	59	75
			79	106	207	88
			76	99	173	76
			89	101	201	75
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	5.2	93	98	229	82	95
			73	87	237	108	95
		6.6	75	89	286	128	125*
			76	81	278	122	125*
		8.2	85	97	300	118	112
			80	82	256	106	112
		10.2	80	82	280	117	113
			78	77	253	108	113
		12.8	98	90	364	124	144*
			72	68	353	165	144*
		16	72	42	475	220	230*
			63	43	449	239	230*
		20	65	22	633	324	299*
			67	26	551	273	299*
Positive Control	3-Methylcholanthrene	5	57	59	570	335	304*
			67	67	595	297
			70	74	591	280

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	109	291	102	97
			86	99	268	103
			97	112	278	95
			77	80	201	87
Test Chemical	4,4'-Methylenebis(N,N-dimethyl)benzenamine	8.1	89	95	368	138	165*
			81	82	466	192	165*
		10.2	64	59	447	233
		12.7	73	50	546	251	229*
			80	56	495	207	229*
		15.9	67	35	507	252	285*
			55	27	527	318	285*
		19.8	58	18	571	328	340*
			52	16	546	352	340*
		24.8	44	9	473	356	382*
			48	9	591	408	382*
		31

Positive Control	3-Methylcholanthrene	5	56	41	625	375	396*
			49	34	535	368
			51	40	685	446

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination