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Study Information
Study Attribute Value
CEBS Accession Number: 002-01467-0001-0000-1
Chemical Name: 4,4'-Methylenedianiline dihydrochloride
CASRN: 13552-44-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 204381_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 04/23/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1046 426 0.407 8.52 26.5 0.000
Test Chemical 4,4'-Methylenedianiline dihydrochloride 
5         
 50 1049 419 0.399 8.38 26.5 -1.924 
16         
 50 1046 530 0.507 10.60 26.5 24.413 
50         
 50 1049 555 0.529 11.10 26.5 29.909 
160         
 50 1048 579 0.552 11.58 26.5 35.656 
500         
 0 0 0 0.000 0.00 26.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1050 577 0.550 11.54 26.5 34.930
Mitomycin-C 
 0.005     
 10 210 232 1.105 23.20 26.5 171.263
Trend: 6.216        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 05/21/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1044 406 0.389 8.12 26.5 0.000
Test Chemical 4,4'-Methylenedianiline dihydrochloride 
50         
 50 1040 474 0.456 9.48 26.5 17.198 
100         
 50 1040 495 0.476 9.90 26.5 22.390 
160         
 50 1046 528 0.505 10.56 31.5 29.801 
300         
 50 1045 498 0.477 9.96 36.5 22.543 
500         
 50 1046 551 0.527 11.02 36.5 35.455
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 511 0.487 10.22 26.5 25.262
Mitomycin-C 
 0.005     
 10 210 239 1.138 23.90 26.5 192.653
Trend: 4.393        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/23/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1044 410 0.393 8.20 26.0 0.000
Test Chemical 4,4'-Methylenedianiline dihydrochloride 
50         
 50 1041 448 0.430 8.96 26.0 9.583 
160         
 50 1040 494 0.475 9.88 26.0 20.951 
500         
 50 1045 494 0.473 9.88 26.0 20.373 
1600         
 50 1045 515 0.493 10.30 26.0 25.490
Positive Control Cyclophosphamide 
 0.3       
 50 1044 898 0.860 17.96 26.0 119.024
Cyclophosphamide 
 0.6       
 10 210 275 1.310 27.50 26.0 233.449
Trend: 3.687        
Probability: 0.000