Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01467-0011-0000-2
Chemical Name:	4,4'-Methylenedianiline dihydrochloride
CASRN:	13552-44-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	641032
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	69	106	79	38	45
			75	99	107	48
			78	92	113	49
			74	104	98	44
Test Chemical	4,4'-Methylenedianiline dihydrochloride	125	68	75	97	48	50
			56	76	88	53	50
		250	56	70	118	70	74*
			50	53	117	78	74*
		500	67	49	197	98	95*
			65	52	180	92	95*
		1000

Positive Control	Methyl Methane Sulfonate	15	35	30	191	184	202*
			29	21	193	221	202*
	Methyl Methane Sulfonate	250	71	76	498	235	232*
			72	87	491	228	232*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	79	96	83	35	38
			76	103	88	39
			73	99	92	42
			78	102	85	36
Test Chemical	4,4'-Methylenedianiline dihydrochloride	78.125	75	98	93	42	42
			79	91	100	42	42
		156.25	96	94	117	41	41
			78	90	96	41	41
		312.5	70	76	73	35	31
			78	90	66	28	31
		625	61	49	123	68	60
			74	56	117	53	60
		1250

Positive Control	Methyl Methane Sulfonate	15	41	26	124	101	110*
			45	31	161	119	110*
	Methyl Methane Sulfonate	250	41	29	135	111	113*
			37	26	130	116	113*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	90	93	106	39	42
			93	95	150	54
			94	107	101	36
			87	105	102	39
Test Chemical	4,4'-Methylenedianiline dihydrochloride	100	100	82	174	58	61
			91	87	175	64	61
		400	66	58	158	80	68*
			101	67	168	56	68*
		700	58	32	231	132	123*
			72	30	243	113	123*
		1000

Positive Control	Methyl Methane Sulfonate	15	45	40	151	113	123*
			47	34	187	132	123*
	Methyl Methane Sulfonate	250	81	72	326	134	150*
			73	68	360	166	150*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination