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Study Information
Study Attribute Value
CEBS Accession Number: 002-01467-0011-0000-2
Chemical Name: 4,4'-Methylenedianiline dihydrochloride
CASRN: 13552-44-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 641032
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 69 106 79 38 45
    75 99 107 48
    78 92 113 49
    74 104 98 44
Test Chemical 4,4'-Methylenedianiline dihydrochloride 
125         
 68 75 97 48 50
    56 76 88 53
  
250         
 56 70 118 70 74*
    50 53 117 78
  
500         
 67 49 197 98 95*
    65 52 180 92
  
1000         
   
Positive Control Methyl Methane Sulfonate 
15         
 35 30 191 184 202*
    29 21 193 221
Methyl Methane Sulfonate 
250         
 71 76 498 235 232*
    72 87 491 228

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 79 96 83 35 38
    76 103 88 39
    73 99 92 42
    78 102 85 36
Test Chemical 4,4'-Methylenedianiline dihydrochloride 
78.125     
 75 98 93 42 42
    79 91 100 42
  
156.25      
 96 94 117 41 41
    78 90 96 41
  
312.5       
 70 76 73 35 31
    78 90 66 28
  
625         
 61 49 123 68 60
    74 56 117 53
  
1250         
   
Positive Control Methyl Methane Sulfonate 
15         
 41 26 124 101 110*
    45 31 161 119
Methyl Methane Sulfonate 
250         
 41 29 135 111 113*
    37 26 130 116

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 90 93 106 39 42
    93 95 150 54
    94 107 101 36
    87 105 102 39
Test Chemical 4,4'-Methylenedianiline dihydrochloride 
100         
 100 82 174 58 61
    91 87 175 64
  
400         
 66 58 158 80 68*
    101 67 168 56
  
700         
 58 32 231 132 123*
    72 30 243 113
  
1000         
   
Positive Control Methyl Methane Sulfonate 
15         
 45 40 151 113 123*
    47 34 187 132
Methyl Methane Sulfonate 
250         
 81 72 326 134 150*
    73 68 360 166

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination