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Study Information
Study Attribute Value
CEBS Accession Number: 002-01469-0011-0000-4
Chemical Name: 4,4'-Sulfonyldianiline (Dapsone)
CASRN: 80-08-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 001410
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 105# 87 115 37 43
    99 108 126 42
    104 105 110 35
    99 100 165 56
Test Chemical 4,4'-Sulfonyldianiline (Dapsone) 
125         
 107# 98 138 43 41
    97# 94 115.5 40
  
250         
 107 95 151 47 45
    99 73 127 43
  
500         
 93 69 155 56 65
    92 63 203 74
  
1000         
 113 13 129 38 65
    99 39 274 93
  
2000         
 87# 6 123 47 48
    98 10 141 48
Positive Control Methyl Methane Sulfonate 
15         
 38 25 458 405 395*
    40# 22 462 385

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 58 93 156 89 74
    70 101 160 76
    69 106 120 58
    47r 61 138 98
Test Chemical 4,4'-Sulfonyldianiline (Dapsone) 
800         
 77 28 111 48 43
    87 47 99 38
  
1200         
 87# 52 258 99 70
    79 31 98 41
  
1600         
 95 23 86 30 24
    88 16 48 18
  
2000         
 82 21 95 39 30
    84 20 53 21
  
2400         
 128r 15 91 24
    73# 16 69 31
Positive Control Methyl Methane Sulfonate 
15         
 24 23 173 240 307*
    21 22 230 374

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 112# 93 100.5 30 41
    111 91 146 44
    98 125 140 47
    91# 92 121.5 45
Test Chemical 4,4'-Sulfonyldianiline (Dapsone) 
100         
 87# 158 110 42 46
    89 118 132 49
  
200         
 98 136 153 52 40
    85 144 71 28
  
400         
 103 120 120 39 46
    101# 106 162 53
  
800         
 72 79 205 95 92*
    79 86 213 89
  
1600         
   
Positive Control Methyl Methane Sulfonate 
15         
 46# 35 416 300 321*
    38# 35 387 342

Nonactivation Trial: 4 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 99 143 54 61
    103 102 203 66
    99 103 159 53
    92 96 189 69
Test Chemical 4,4'-Sulfonyldianiline (Dapsone) 
100         
 77 97 178 77 66
    89 104 146 55
  
200         
 105 119 181 57 60
    105 107 199 63
  
400         
 91 97 136 50 57
    109 104 209 64
  
800         
 91 22 128 47 52
    97 23 163 56
  
1600         
 92 15 177 64 62
    92 12 166 60
Positive Control Methyl Methane Sulfonate 
15         
 60 42 321 178 167*
    65 49 308 157

Nonactivation Trial: 5 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 94 78 34 33
    77 83 51 22
    96 112 120 42
    103 110 105 34
Test Chemical 4,4'-Sulfonyldianiline (Dapsone) 
100         
 70 106 112 53 50
    79 93 110 46
  
200         
 81 96 116 48 50
    76 82 118 52
  
400         
 79 92 118 50 53*
    81 81 134 55
  
800         
 91 16 98 36 39
    88 13 109 41
  
1600         
 83 13 96 38 43
    74 12 104 47
Positive Control Methyl Methane Sulfonate 
15         
 57 40 216 127 134*
    52 37 220 142

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 54 87 122 76 74
    62 98 106 57
    73# 110 162 74
    67 105 175 87
Test Chemical 4,4'-Sulfonyldianiline (Dapsone) 
100         
 91 105 165 61 65
    77 108 158 69
  
300         
 71 84 176 82 76
    75 87 158 70
  
500         
 73 78 165 75 71
    85 80 168 66
  
700         
 68 59 192 94 86
    73 33 172 78
  
1000         
 73 13 197 90 91
    75 13 207 92
Positive Control 3-Methylcholanthrene 
2.5       
 66 57 587 297 304*
    66 47 614 310

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 70 91 38 18 22
    56 94 46 27
    69 107 36 17
    74 108 57 26
Test Chemical 4,4'-Sulfonyldianiline (Dapsone) 
300         
 71 92 39 18 18
    77 79 41 18
  
400         
 84 115 46 18 21
    83 104 57 23
  
500         
 94 93 265 94 57*
    85 97 49 19
  
600         
 84 29 68 27 20
    83 90 33 13
  
700         
 71 69 42 20 22
    73 78 54 25
Positive Control 3-Methylcholanthrene 
2.5       
 47 26 378 268 265*
    40 28 316 261

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination