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Study Information
Study Attribute Value
CEBS Accession Number: 002-01472-0001-0000-7
Chemical Name: 4,4-Thiobis(6-tert-butyl-m-cresol)
CASRN: 96-69-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 395386_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Questionable 
No Activation  3  Weakly Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 12/06/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1041 540 0.519 10.80 26.0 0.000
Test Chemical 4,4-Thiobis(6-tert-butyl-m-cresol) 
 0.5       
 50 1046 511 0.489 10.22 26.0 -5.823 
1.67      
 0 0 0 0.000 0.00 33.5 N/A
Positive Control Mitomycin-C 
 0.0015    
 50 1048 830 0.792 16.60 26.0 52.677
Mitomycin-C 
 0.01      
 5 104 248 2.385 49.60 26.0 359.701
Trend: -0.972        
Probability: 0.835        

Trial #: 2    Activation: No Activation    Date: 01/24/1984    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 512 0.491 10.24 26.0 0.000
Test Chemical 4,4-Thiobis(6-tert-butyl-m-cresol) 
1.75      
 50 1039 527 0.507 10.54 26.0 3.326 
2         
 50 1036 526 0.508 10.52 26.0 3.429 
2.5       
 50 1048 602 0.574 12.04 31.5 17.017
Positive Control Mitomycin-C 
 0.0015    
 50 1049 805 0.767 16.10 26.0 56.327
Mitomycin-C 
 0.01      
 5 105 280 2.667 56.00 26.0 443.229
Trend: 2.636        
Probability: 0.004        

Trial #: 3    Activation: No Activation    Date: 02/28/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 477 0.454 9.54 26.0 0.000
Test Chemical 4,4-Thiobis(6-tert-butyl-m-cresol) 
1.5       
 50 1047 551 0.526 11.02 26.0 15.955 
2         
 50 1050 492 0.469 9.84 26.0 3.243 
2.5       
 50 1050 578 0.550 11.56 33.5 21.289 
5         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0015    
 50 1046 815 0.779 16.30 26.0 71.676
Mitomycin-C 
 0.01      
 5 105 237 2.257 47.40 26.0 397.329
Trend: 2.376        
Probability: 0.009        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 12/06/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 551 0.526 11.02 26.0 0.000
Test Chemical 4,4-Thiobis(6-tert-butyl-m-cresol) 
5         
 50 1050 505 0.481 10.10 26.0 -8.610 
16.7       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1044 989 0.947 19.78 26.0 80.008
Cyclophosphamide 
2         
 5 104 210 2.019 42.00 26.0 283.690
Trend: -1.462        
Probability: 0.928        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 01/24/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 583 0.560 11.66 26.0 0.000
Test Chemical 4,4-Thiobis(6-tert-butyl-m-cresol) 
7.5       
 50 1044 741 0.710 14.82 31.5 26.858 
10         
 50 1045 741 0.709 14.82 31.5 26.736 
12.5       
 50 1044 820 0.785 16.40 34.3 40.382 
15         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1043 866 0.830 17.32 26.0 48.400
Cyclophosphamide 
2         
 5 105 249 2.371 49.80 26.0 323.847
Trend: 5.789        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 02/28/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 471 0.451 9.42 26.0 0.000
Test Chemical 4,4-Thiobis(6-tert-butyl-m-cresol) 
7.5       
 50 1047 519 0.496 10.38 26.0 9.875 
10         
 50 1050 574 0.547 11.48 33.5 21.172 
12.5       
 50 1050 625 0.595 12.50 33.5 31.938 
15         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1049 750 0.715 15.00 26.0 58.477
Cyclophosphamide 
2         
 5 105 199 1.895 39.80 26.0 320.091
Trend: 4.836        
Probability: 0.000