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Study Information
Study Attribute Value
CEBS Accession Number: 002-01478-0002-0000-4
Chemical Name: 4-(Chloroacetyl)acetanilide
CASRN: 140-49-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 223043_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive* 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Weakly Positive* 
Induced Rat Liver S9  3_S9  Negative 

Trial #:1   Activation: No Activation    Date: 09/26/1984    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4-(Chloroacetyl)acetanilide 
 0.5       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2.5       
 100 13 0.130 11.0 4 0.040 3.0 9 0.090 8.0 0 0.000 0.0 
5         
 44 14 0.318 25.0 1 0.023 2.0 12 0.273 23.0 1 0.023 2.0 
16         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 27 0.270 21.0 19 0.190 14.0 8 0.080 8.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 14 0.280 28.0 12 0.240 24.0 2 0.040 4.0 0 0.000 0.0
Trend: 6.496 2.311 6.038  
Probability: 0.000 0.010 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 12/05/1984    Harvest Time: 14 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4-(Chloroacetyl)acetanilide 
 0.5       
 100 3 0.030 3.0 1 0.010 1.0 1 0.010 1.0 1 0.010 1.0 
1         
 100 4 0.040 4.0 1 0.010 1.0 2 0.020 2.0 1 0.010 1.0 
2.5       
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
5         
 100 18 0.180 15.0 6 0.060 6.0 12 0.120 11.0 0 0.000 0.0 
7.5       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 48 0.480 35.0 19 0.190 16.0 28 0.280 21.0 1 0.010 1.0
Trend: 4.307 2.942 4.045  
Probability: 0.000 0.002 0.000  


Trial #:2   Activation: No Activation    Date: 11/28/1984    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 4-(Chloroacetyl)acetanilide 
 0.1       
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
 0.5       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1         
 100 4 0.040 4.0 1 0.010 1.0 1 0.010 1.0 2 0.020 2.0 
2.5       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 20 0.200 16.0 11 0.110 11.0 9 0.090 7.0 0 0.000 0.0
Trend: 1.204 1.203 -0.393  
Probability: 0.114 0.114 0.653  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 09/26/1984    Harvest Time: 14 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4-(Chloroacetyl)acetanilide 
1         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2.5       
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
5         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
10         
 100 46 0.460 29.0 30 0.300 20.0 14 0.140 11.0 2 0.020 2.0
Positive Control: Cyclophosphamide 
15         
 100 35 0.350 32.0 20 0.200 18.0 15 0.150 14.0 0 0.000 0.0
Trend: 7.083 5.767 4.393  
Probability: 0.000 0.000 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 11/28/1984    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: 4-(Chloroacetyl)acetanilide 
1         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
2.5       
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
5         
 100 9 0.090 5.0 2 0.020 2.0 6 0.060 3.0 1 0.010 1.0 
7.5       
 100 8 0.080 6.0 2 0.020 1.0 6 0.060 5.0 0 0.000 0.0 
10         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 10 0.100 10.0 5 0.050 5.0 5 0.050 5.0 0 0.000 0.0
Trend: 1.481 1.709 0.844  
Probability: 0.069 0.044 0.199