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Study Information
Study Attribute Value
CEBS Accession Number: 002-01481-0001-0000-7
Chemical Name: 4-Acetylaminofluorene
CASRN: 28322-02-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 710698_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 07/15/1987    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 462 0.446 9.24 25.3 0.000
Test Chemical 4-Acetylaminofluorene 
100         
 50 1042 508 0.488 10.16 30.3 9.324 
333.3       
 50 1046 544 0.520 10.88 30.3 16.623 
1000         
 50 1040 496 0.477 9.92 30.3 6.946
Positive Control Mitomycin-C 
 0.001     
 50 1040 622 0.598 12.44 25.3 34.114
Mitomycin-C 
 0.01      
 5 105 289 2.752 57.80 25.3 517.201
Trend: 1.330        
Probability: 0.092        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/14/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1250 378 0.302 7.56 25.3 0.000
Test Chemical 4-Acetylaminofluorene 
33.3       
 50 1050 406 0.387 8.12 25.3 27.866 
100         
 50 1044 417 0.399 8.34 25.3 32.085 
333.3       
 50 1040 652 0.627 13.04 25.3 107.316 
1000         
 50 1049 535 0.510 10.70 25.3 68.654
Positive Control Cyclophosphamide 
 0.4       
 50 1044 624 0.598 12.48 25.3 97.652
Cyclophosphamide 
2         
 5 105 170 1.619 34.00 25.3 435.399
Trend: 10.595        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/12/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 524 211 0.403 8.44 25.7 0.000
Negative (Not Specified) 
0         
 25 524 287 0.548 11.48 25.7 36.019
Test Chemical 4-Acetylaminofluorene 
333         
 25 525 285 0.543 11.40 25.7 34.814 
500         
 25 519 283 0.545 11.32 25.7 35.415 
750         
 25 524 324 0.618 12.96 25.7 53.555 
1000         
 25 522 291 0.557 11.64 25.7 38.443
Positive Control Cyclophosphamide 
 0.4       
 25 524 338 0.645 13.52 25.7 60.190
Cyclophosphamide 
2         
 5 103 156 1.515 31.20 25.7 276.128
Trend: 3.921        
Probability: 0.000