Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01481-0003-0000-9
Chemical Name:	4-Acetylaminofluorene
CASRN:	28322-02-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	390698
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	145r	107	134	31	33
			88	120	85	32
			99	81	97	33
			98	99	99	34
Test Chemical	4-Acetylaminofluorene	31.3	80	62	106	44	34
			91	63	84	31
			88	83	69	26
		62.5	86	52	65	25	22
			116	71	85	24
			114	65	54	16
		125	109	40	134	41	41
			76	39	95	42
			82	43	99	40
		250	96	49	119	41	35
			101	51	96	32
			101	42	96	32
		500	106	25	45	14	20
			79	30	44	18
			93	33	78	28
		1000	107	19	70	22	25
			117	17	89	25
			89	15	72	27
Positive Control	Methyl Methane Sulfonate	5	98	54	508	172	182*
			107	69	576	179
			95	47	555	194

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	91	62	26	29
			85	100	82	32
			78	93	73	31
			85#	115	68	27
Test Chemical	4-Acetylaminofluorene	31.3	76#	75	50	22	20
			75	75	49	22
			87#	91	43	16
		62.5	67	61	45	22	23
			57	58	50	29
			73#	97	39	18
		125	71	49	45	21	26
			63	44	48	25
			67	45	63	31
		250	82	35	55	22	27
			76#	39	75	33
			96	69	72	25
		500	99	28	69	23	23
			70	20	46	22
			87#	49	59	23
		1000	77	17	57	25	20
			73	19	39	18
			68	12	33	16
Positive Control	Methyl Methane Sulfonate	5	62	48	504	273	256*
			67	60	444	221
			55	51	447	273

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	109	69	37	34
			67#	101	66	33
			64#	83	59	31
			95#	107	100	35
Test Chemical	4-Acetylaminofluorene	15.6	101	145	97	32	38
			92	83	119	43
			84	76	101	40
		31.3	94	101	130	46	38
			108	112	100	31
			94	85	104	37
		62.5	82	61	205	84	73*
			89	47	166	63
			123r	76	204	55
		125	80	44	312	130	119*
			93	54	306	109
			90	32	318	118
		250	74	37	288	130	147*
			83	7	361	145
			77	46	381	166
		500


Positive Control	3-Methylcholanthrene	2.5	60#	55	460	257	213*
			82	60	537	217
			85	71	418	165

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75#	108	52	23	23
			63	77	44	23
			65	82	31	16
			86	133	75	29
Test Chemical	4-Acetylaminofluorene	100	52	28	44	28	54*
			47	29	91	65
			55	31	111	67
		150	59	26	159	90	84*
			60	28	144	80
			52	26	128	82
		200	58	19	150	86	78*
			65	25	159	82
			73	26	144	66
		300	65	22	164	84	81*
			74	24	176	79
			84	27	204	81
		400	74	19	182	82	87*
			79	19	227	96
			64	19	161	84
		500	68	14	224	110	102*
			78	13	230	98
			79	10	233	98
Positive Control	3-Methylcholanthrene	2.5	38	7	426	377	361*
			31	6	318	338
			32	7	356	367

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination