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Study Information
Study Attribute Value
CEBS Accession Number: 002-01481-0003-0000-9
Chemical Name: 4-Acetylaminofluorene
CASRN: 28322-02-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 390698
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 145r 107 134 31 33
    88 120 85 32
    99 81 97 33
    98 99 99 34
Test Chemical 4-Acetylaminofluorene 
31.3       
 80 62 106 44 34
    91 63 84 31
    88 83 69 26
  
62.5       
 86 52 65 25 22
    116 71 85 24
    114 65 54 16
  
125         
 109 40 134 41 41
    76 39 95 42
    82 43 99 40
  
250         
 96 49 119 41 35
    101 51 96 32
    101 42 96 32
  
500         
 106 25 45 14 20
    79 30 44 18
    93 33 78 28
  
1000         
 107 19 70 22 25
    117 17 89 25
    89 15 72 27
Positive Control Methyl Methane Sulfonate 
5         
 98 54 508 172 182*
    107 69 576 179
    95 47 555 194

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 91 62 26 29
    85 100 82 32
    78 93 73 31
    85# 115 68 27
Test Chemical 4-Acetylaminofluorene 
31.3       
 76# 75 50 22 20
    75 75 49 22
    87# 91 43 16
  
62.5       
 67 61 45 22 23
    57 58 50 29
    73# 97 39 18
  
125         
 71 49 45 21 26
    63 44 48 25
    67 45 63 31
  
250         
 82 35 55 22 27
    76# 39 75 33
    96 69 72 25
  
500         
 99 28 69 23 23
    70 20 46 22
    87# 49 59 23
  
1000         
 77 17 57 25 20
    73 19 39 18
    68 12 33 16
Positive Control Methyl Methane Sulfonate 
5         
 62 48 504 273 256*
    67 60 444 221
    55 51 447 273

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 63 109 69 37 34
    67# 101 66 33
    64# 83 59 31
    95# 107 100 35
Test Chemical 4-Acetylaminofluorene 
15.6       
 101 145 97 32 38
    92 83 119 43
    84 76 101 40
  
31.3       
 94 101 130 46 38
    108 112 100 31
    94 85 104 37
  
62.5       
 82 61 205 84 73*
    89 47 166 63
    123r 76 204 55
  
125         
 80 44 312 130 119*
    93 54 306 109
    90 32 318 118
  
250         
 74 37 288 130 147*
    83 7 361 145
    77 46 381 166
  
500         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 60# 55 460 257 213*
    82 60 537 217
    85 71 418 165

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75# 108 52 23 23
    63 77 44 23
    65 82 31 16
    86 133 75 29
Test Chemical 4-Acetylaminofluorene 
100         
 52 28 44 28 54*
    47 29 91 65
    55 31 111 67
  
150         
 59 26 159 90 84*
    60 28 144 80
    52 26 128 82
  
200         
 58 19 150 86 78*
    65 25 159 82
    73 26 144 66
  
300         
 65 22 164 84 81*
    74 24 176 79
    84 27 204 81
  
400         
 74 19 182 82 87*
    79 19 227 96
    64 19 161 84
  
500         
 68 14 224 110 102*
    78 13 230 98
    79 10 233 98
Positive Control 3-Methylcholanthrene 
2.5       
 38 7 426 377 361*
    31 6 318 338
    32 7 356 367

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination