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Study Information
Study Attribute Value
CEBS Accession Number: 002-01481-0004-0000-0
Chemical Name: 4-Acetylaminofluorene
CASRN: 28322-02-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 888359
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 94 173 81 62
    91 106 117 43
Test Chemical 4-Acetylaminofluorene 
125         
 70 76 120 57
  
250         
 62 48 102 55 63
    68 49 146 72
  
500         
 66 25 95 48 56
    79 35 150 63
  
1000         
 65 22 126 65 66
    70 19 141 68
  
2000         
 80 21 145 60 67
    76 11 169 74
Positive Control Ethyl Methane Sulfonate 
250         
 52 56 648 411 411*

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 106 158 60 49
    95 126 119 42
    67 81 73 36
    58 86 99 57
Test Chemical 4-Acetylaminofluorene 
62.5       
 64 84 118 61 57
    67 107 107 53
  
125         
 83 57 172 69 58
    71 73 99 46
  
250         
 70 44 141 67 76
    49 48 124 84
  
500         
 76 33 193 85 83*
    76 29 184 81
  
1000         
 59 14 214 121 98*
    75 17 167 74
Positive Control Ethyl Methane Sulfonate 
250         
 59 73 636 357 344*
    69 67 682 330

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 51 97 158 103 94
    47r 103 127 90
    48r 94 163 112
    70 103 179 86
Test Chemical 4-Acetylaminofluorene 
200         
 44 43 187 141 126
    57 57 211 124
    58 53 198 113
  
400         
 59 43 197 112 97
    70 55 154 73
    55 43 176 107
  
600         
 52 24 320 203 147*
    66 36 299 152
    63 41 160 84
  
800         
 54 31 186 116 94
    77 19 230 100
    66 19 132 67
  
1000         
 62 14 243 131 100
    68 28 120 59
    69 25 229 111
Positive Control Ethyl Methane Sulfonate 
250         
 49 82 511 349 353*
    45 78 482 357

Non-Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 146 119 44 76
    72 84 201 94
    66 91 138 70
    65 79 192 98
Test Chemical 4-Acetylaminofluorene 
62.5       
 54 88 179 111 106
    58 87 178 101
  
125         
 55 82 174 106 108
    67 83 218 109
  
250         
 56 47 175 104 112
    59 51 213 120
  
500         
 48 29 168 117 111
    58 25 184 105
  
1000         
 46 10 186 134 115
    67 13 193 96
Positive Control 3-Methylcholanthrene 
2.5       
 69 59 349 168 162*
    60 80 277 155

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 103 118 53 62
    87 91 172 66
    90 103 166 61
    83 104 167 67
Test Chemical 4-Acetylaminofluorene 
50         
 79 97 193 81 75
    102 113 210 69
  
100         
 84 82 206 82 72
    83 115 156 63
  
200         
 90 86 179 66 65
    100 71 191 64
  
400         
 67 51 133 66 76
    68 37 174 86
  
800         
 71 22 163 77 84
    74 18 201 91
Positive Control 3-Methylcholanthrene 
2.5       
 66 40 576 292 301*
    62 39 576 311

Induced S9 Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 44r 61 317 238 49
    75 122 107 47
    50 91 71 47
    64 87 101 53
Test Chemical 4-Acetylaminofluorene 
200         
 55 114 71 43 44
    83# 93 113 46
  
400         
 58 71 174 100 88*
    75 95 168 75
  
600         
 87 25 169 65 67
    68 34 143 70
  
800         
 66 23 162 82 72
    68 17 127 62
  
1000         
 95 24 167 59 63
    83 18 164 66
Positive Control 3-Methylcholanthrene 
2.5       
 41 43 468 377 442*
    30 35 453 506

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination