Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01481-0004-0000-0
Chemical Name:	4-Acetylaminofluorene
CASRN:	28322-02-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	888359
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	94	173	81	62
Vehicle Control			91	106	117	43	62
Test Chemical	4-Acetylaminofluorene	125	70	76	120	57
		250	62	48	102	55	63
			68	49	146	72	63
		500	66	25	95	48	56
			79	35	150	63	56
		1000	65	22	126	65	66
			70	19	141	68	66
		2000	80	21	145	60	67
			76	11	169	74	67
Positive Control	Ethyl Methane Sulfonate	250	52	56	648	411	411*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	106	158	60	49
			95	126	119	42
			67	81	73	36
			58	86	99	57
Test Chemical	4-Acetylaminofluorene	62.5	64	84	118	61	57
			67	107	107	53	57
		125	83	57	172	69	58
			71	73	99	46	58
		250	70	44	141	67	76
			49	48	124	84	76
		500	76	33	193	85	83*
			76	29	184	81	83*
		1000	59	14	214	121	98*
			75	17	167	74	98*
Positive Control	Ethyl Methane Sulfonate	250	59	73	636	357	344*
Positive Control			69	67	682	330	344*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	51	97	158	103	94
			47r	103	127	90
			48r	94	163	112
			70	103	179	86
Test Chemical	4-Acetylaminofluorene	200	44	43	187	141	126
			57	57	211	124
			58	53	198	113
		400	59	43	197	112	97
			70	55	154	73
			55	43	176	107
		600	52	24	320	203	147*
			66	36	299	152
			63	41	160	84
		800	54	31	186	116	94
			77	19	230	100
			66	19	132	67
		1000	62	14	243	131	100
			68	28	120	59
			69	25	229	111
Positive Control	Ethyl Methane Sulfonate	250	49	82	511	349	353*
Positive Control			45	78	482	357	353*

Non-Induced S9 Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	146	119	44	76
			72	84	201	94
			66	91	138	70
			65	79	192	98
Test Chemical	4-Acetylaminofluorene	62.5	54	88	179	111	106
			58	87	178	101	106
		125	55	82	174	106	108
			67	83	218	109	108
		250	56	47	175	104	112
			59	51	213	120	112
		500	48	29	168	117	111
			58	25	184	105	111
		1000	46	10	186	134	115
			67	13	193	96	115
Positive Control	3-Methylcholanthrene	2.5	69	59	349	168	162*
Positive Control			60	80	277	155	162*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	103	118	53	62
			87	91	172	66
			90	103	166	61
			83	104	167	67
Test Chemical	4-Acetylaminofluorene	50	79	97	193	81	75
			102	113	210	69	75
		100	84	82	206	82	72
			83	115	156	63	72
		200	90	86	179	66	65
			100	71	191	64	65
		400	67	51	133	66	76
			68	37	174	86	76
		800	71	22	163	77	84
			74	18	201	91	84
Positive Control	3-Methylcholanthrene	2.5	66	40	576	292	301*
Positive Control			62	39	576	311	301*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	44r	61	317	238	49
			75	122	107	47
			50	91	71	47
			64	87	101	53
Test Chemical	4-Acetylaminofluorene	200	55	114	71	43	44
			83#	93	113	46	44
		400	58	71	174	100	88*
			75	95	168	75	88*
		600	87	25	169	65	67
			68	34	143	70	67
		800	66	23	162	82	72
			68	17	127	62	72
		1000	95	24	167	59	63
			83	18	164	66	63
Positive Control	3-Methylcholanthrene	2.5	41	43	468	377	442*
Positive Control			30	35	453	506	442*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination