Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01481-0005-0000-1
Chemical Name:	4-Acetylaminofluorene
CASRN:	28322-02-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G12606
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	92	146	66	70
Vehicle Control			78	108	172	73	70

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	107	44	19	19
Vehicle Control			73	92	44	20	19
Test Chemical	4-Acetylaminofluorene	31.25	64	70	48	25	24
			67	67	46	23	24
		62.5	70	72	45	21	21
			66	66	40	20	21
		125	85	95	54	21	20
			83	83	46	18	20
		250	72	55	26	12	17
			72	62	46	21	17
		500	91	28	67	24	19
			62	20	25	14	19
		1000	78	14	48	21	21
			96	20	64	22	21
Positive Control	Ethyl Methane Sulfonate	500	56	38	947	564	587*
Positive Control			42	31	772	610	587*
Negative Control	Negative (Not Specified)	0	69	100	33	16	17*
Negative Control			86	101	46	18	17*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	68	57	50	25	16
			69	121	28	13
			76	121	22	10
			50r	102	20	13
Test Chemical	4-Acetylaminofluorene	12.5	58	88	35	20	17
			67	98	37	18
			63	109	24	13
		25	61	105	26	14	16
			69	108	32	15
			52	97	30	19
		50	58	72	35	20	19
			67	101	40	20
			58	99	31	18
		100	60	69	22	12	11
			60	85	17	9
			52	74	17	11
		200	65	58	25	13	18
			59	59	32	18
			42	37	27	22
Positive Control	Ethyl Methane Sulfonate	500	27	23	539	665	738*
			21	17	595	944
			31	33	566	605

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	104	122	233	75	79
Vehicle Control			85	89	210	83	79
Test Chemical	4-Acetylaminofluorene	7.82	101	50	282	94	94
			98	48	276	94	94
		15.6	89	31	276	103	106
			91	40	297	108	106
		31.25	90	21	328	122	119
			90	25	313	116	119
		62.5	64	8	358	188	164*
			78	9	326	139	164*
Positive Control	Dimethylnitrosoamine	10	8r	1	251	1091
Negative Control	Negative (Not Specified)	0	92	87	224	81	79*
Negative Control			101	102	234	77	79*

Non-Induced S9 Trial: 2 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	76	145	74	70
Vehicle Control			90	100	181	67	70
Test Chemical	4-Acetylaminofluorene	20	63#	92	147	78	82
			75	65	194	86	82
		40	167r	130	362	72
			62	43	146	78
		60	111	77	264	79
			133r	73	347	87
		80	130r	88	291	75
		100	112	77	311	93	98
			101	45	312	103	98
Positive Control	Dimethylnitrosoamine	10	6r	3	42	233	400*
Positive Control			11	5	136	400	400*
Negative Control	Negative (Not Specified)	0	93	111	161	58	60*
Negative Control			79#	113	150	63	60*

Non-Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	97#	98	131	45	42
Vehicle Control			100	112	116	39	42
Test Chemical	4-Acetylaminofluorene	80	92	12	211	77	75*
			70	18	152	73	75*
		100	84	18	192	76	80*
			64	12	159	83	80*
		125	77	13	149	65	97*
			50	8	194	130	97*
		200	68	9	188	92	87*
			85#	15	208	82	87*
		250	70	11	161	77	76*
			89	11	198	74	76*
Positive Control	3-Methylcholanthrene	7	110#	48	675	204	217*
Positive Control			108#	49	740	229	217*
Negative Control	Negative (Not Specified)	0	82#	90	85.5	35

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	83	135	59	71
Vehicle Control			74	116	187	84	71
Test Chemical	4-Acetylaminofluorene	31.3	89	80	176	66	76
			59	54	153	86	76
		62.5	65	36	144	73	72
			68	37	143	70	72
		125	88	23	333	126	129*
			54	22	215	132	129*
		200	87	15	502	193	190*
			86#	15	481	186	190*
Positive Control	Dimethylnitrosoamine	100	17	7	184	368	361*
Positive Control			21	9	220	355	361*
Negative Control	Negative (Not Specified)	0	74	108	138	62	72*
Negative Control			73	93	177	81	72*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80#	106	174	73
Test Chemical	4-Acetylaminofluorene	40	78#	51	145.5	63	80
			31	31	91	98	80
		60	56	25	175	104	103
			58#	37	176	102	103
		80	48	24	225	155	153*
			60	26	270	150	153*
		100	55	19	405	245	238*
			53	22	369	231	238*
		125	45	12	224	167	177*
			38	11	210	187	177*
Positive Control	3-Methylcholanthrene	5	34	15	385	377	350*
Positive Control			40	12	390	322	350*
Negative Control	Negative (Not Specified)	0	62	118	120	65	71*
Negative Control			71	76	161	76	71*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	114	41	16	21
			67	106	34	17
			75	90	52	23
			79	90	70	29
Test Chemical	4-Acetylaminofluorene	50	73	66	59	27	29
			80	64	74	31	29
		75	62	37	66	36	29
			74	52	51	23	29
		100	70	45	98	47	50*
			58	29	93	53	50*
		125	58	29	114	66	72*
			68	35	161	79	72*
		150	65	22	75	39	44*
			56#	41	84	50	44*
		200	68	30	146	72	58*
			84	42	113	45	58*
Positive Control	3-Methylcholanthrene	5	49	38	400	272	288*
Positive Control			47	24	428	305	288*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination