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Study Information
Study Attribute Value
CEBS Accession Number: 002-01481-0005-0000-1
Chemical Name: 4-Acetylaminofluorene
CASRN: 28322-02-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G12606
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 92 146 66 70
    78 108 172 73

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78 107 44 19 19
    73 92 44 20
Test Chemical 4-Acetylaminofluorene 
31.25      
 64 70 48 25 24
    67 67 46 23
  
62.5       
 70 72 45 21 21
    66 66 40 20
  
125         
 85 95 54 21 20
    83 83 46 18
  
250         
 72 55 26 12 17
    72 62 46 21
  
500         
 91 28 67 24 19
    62 20 25 14
  
1000         
 78 14 48 21 21
    96 20 64 22
Positive Control Ethyl Methane Sulfonate 
500         
 56 38 947 564 587*
    42 31 772 610
Negative Control Negative (Not Specified) 
 0         
 69 100 33 16 17*
    86 101 46 18

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 68 57 50 25 16
    69 121 28 13
    76 121 22 10
    50r 102 20 13
Test Chemical 4-Acetylaminofluorene 
12.5       
 58 88 35 20 17
    67 98 37 18
    63 109 24 13
  
25         
 61 105 26 14 16
    69 108 32 15
    52 97 30 19
  
50         
 58 72 35 20 19
    67 101 40 20
    58 99 31 18
  
100         
 60 69 22 12 11
    60 85 17 9
    52 74 17 11
  
200         
 65 58 25 13 18
    59 59 32 18
    42 37 27 22
Positive Control Ethyl Methane Sulfonate 
500         
 27 23 539 665 738*
    21 17 595 944
    31 33 566 605

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 104 122 233 75 79
    85 89 210 83
Test Chemical 4-Acetylaminofluorene 
7.82      
 101 50 282 94 94
    98 48 276 94
  
15.6       
 89 31 276 103 106
    91 40 297 108
  
31.25      
 90 21 328 122 119
    90 25 313 116
  
62.5       
 64 8 358 188 164*
    78 9 326 139
Positive Control Dimethylnitrosoamine 
10         
 8r 1 251 1091
Negative Control Negative (Not Specified) 
 0         
 92 87 224 81 79*
    101 102 234 77

Non-Induced S9 Trial: 2 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 66 76 145 74 70
    90 100 181 67
Test Chemical 4-Acetylaminofluorene 
20         
 63# 92 147 78 82
    75 65 194 86
  
40         
 167r 130 362 72
    62 43 146 78
  
60         
 111 77 264 79
    133r 73 347 87
  
80         
 130r 88 291 75
  
100         
 112 77 311 93 98
    101 45 312 103
Positive Control Dimethylnitrosoamine 
10         
 6r 3 42 233 400*
    11 5 136 400
Negative Control Negative (Not Specified) 
 0         
 93 111 161 58 60*
    79# 113 150 63

Non-Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 97# 98 131 45 42
    100 112 116 39
Test Chemical 4-Acetylaminofluorene 
80         
 92 12 211 77 75*
    70 18 152 73
  
100         
 84 18 192 76 80*
    64 12 159 83
  
125         
 77 13 149 65 97*
    50 8 194 130
  
200         
 68 9 188 92 87*
    85# 15 208 82
  
250         
 70 11 161 77 76*
    89 11 198 74
Positive Control 3-Methylcholanthrene 
7         
 110# 48 675 204 217*
    108# 49 740 229
Negative Control Negative (Not Specified) 
 0         
 82# 90 85.5 35

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 83 135 59 71
    74 116 187 84
Test Chemical 4-Acetylaminofluorene 
31.3       
 89 80 176 66 76
    59 54 153 86
  
62.5       
 65 36 144 73 72
    68 37 143 70
  
125         
 88 23 333 126 129*
    54 22 215 132
  
200         
 87 15 502 193 190*
    86# 15 481 186
Positive Control Dimethylnitrosoamine 
100         
 17 7 184 368 361*
    21 9 220 355
Negative Control Negative (Not Specified) 
 0         
 74 108 138 62 72*
    73 93 177 81

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80# 106 174 73
Test Chemical 4-Acetylaminofluorene 
40         
 78# 51 145.5 63 80
    31 31 91 98
  
60         
 56 25 175 104 103
    58# 37 176 102
  
80         
 48 24 225 155 153*
    60 26 270 150
  
100         
 55 19 405 245 238*
    53 22 369 231
  
125         
 45 12 224 167 177*
    38 11 210 187
Positive Control 3-Methylcholanthrene 
5         
 34 15 385 377 350*
    40 12 390 322
Negative Control Negative (Not Specified) 
 0         
 62 118 120 65 71*
    71 76 161 76

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 114 41 16 21
    67 106 34 17
    75 90 52 23
    79 90 70 29
Test Chemical 4-Acetylaminofluorene 
50         
 73 66 59 27 29
    80 64 74 31
  
75         
 62 37 66 36 29
    74 52 51 23
  
100         
 70 45 98 47 50*
    58 29 93 53
  
125         
 58 29 114 66 72*
    68 35 161 79
  
150         
 65 22 75 39 44*
    56# 41 84 50
  
200         
 68 30 146 72 58*
    84 42 113 45
Positive Control 3-Methylcholanthrene 
5         
 49 38 400 272 288*
    47 24 428 305

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination