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Study Information
Study Attribute Value
CEBS Accession Number: 002-01481-0006-0000-2
Chemical Name: 4-Acetylaminofluorene
CASRN: 28322-02-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G42545
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92 110 234 85 96
    81 90 261 108
Test Chemical 4-Acetylaminofluorene 
77.8       
 61 66 225 122 109
    59 74 167 95
  
130         
 59 53 161 90 80
    66 70 138 70
  
216         
 67 55 225 112 100
    78 68 206 88
  
360         
 56 42 154 92 86
    62 44 149 81
  
600         
 69 53 209 101 98
    76 59 217 95
  
1000         
 68# 51 223 109 103
    63 44 185 97
Positive Control Ethyl Methane Sulfonate 
500         
 36 23 767 720 706*
    41 28 859 693

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75 81 220 98 94
    74 119 198 90
Test Chemical 4-Acetylaminofluorene 
27.7       
 62 89 218 118 111
    66 85 204 104
  
50.3       
 60 80 268 148 135
    57 68 208 122
  
91.5       
 51 41 265 173 165*
    56 52 262 157
  
166         
 64# 32 288 149 142
    57 28 229 135
  
303         
 57 32 290 171 158*
    54 36 237 145
  
550         
 54 33 265 164 138
    63 47 211 112
  
1000         
 50 37 237 158 156*
    65 40 301 155
Positive Control 3-Methylcholanthrene 
7         
 45 48 462 343 294*
    57 59 420 245
3-Methylcholanthrene 
10         
 12 10 104 293 293*
    10r 7 146 503

Non-Induced S9 Trial: 2 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 98 140 54 56
    84 102 147 58
Test Chemical 4-Acetylaminofluorene 
2.4       
 82 111 150 61 58
    78 115 130 56
  
8.1       
 86 129 157 61 50
    91 111 106 39
  
27         
 105 186 137 44 42
    91 170 108 40
  
90         
 94 129 236 83 80
    88 135 202 76
  
300         
 81 88 252 104 102*
    86 89 259 100
  
1000         
 72 69 250 115 115*
    84 87 287 114
Positive Control 3-Methylcholanthrene 
7         
 45 44 401 297 254*
    68 47 433 211

Induced S9 Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 66 94 119 60 49
    71 106 79 37
Test Chemical 4-Acetylaminofluorene 
46.7       
 48 75 144 100
  
77.8       
 77# 109 199 86 93*
    64 91 189 99
  
130         
 56# 55 292.5 175 170*
    63 59 311 166
  
216         
 48 25 388 271 308*
    33 18 341 344
  
360         
 29 6 397 454 461*
    29 9 407 468
  
600         
   
Positive Control 3-Methylcholanthrene 
5         
 43 44 257 199 218*
    39 48 277 238

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 99 121 48 46
    102 101 132 43
Test Chemical 4-Acetylaminofluorene 
46.7       
 90 113 134 50 44
    78 99 89 38
  
77.8       
 79 93 261 111 98*
    82 90 212 86
  
130         
 68 46 283 139 131*
    76 68 279 123
  
216         
 70 28 447 214 207*
    72 40 433 200
  
360         
 65 25 569 290 273*
    63 23 486 256
Positive Control 3-Methylcholanthrene 
5         
 66 60 473 238 227*
    70 64 453 216
3-Methylcholanthrene 
100         
 12 7 93 270 340*
    10 7 123 410

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 121 97 35 34
    85 99 80 31
    93 99 101 36
    87 82 84 32
Test Chemical 4-Acetylaminofluorene 
67         
 95 90 54 19 26
    81 65 79 32
  
84         
 102 91 135 44 35
    108 85 82 25
  
105         
 90 55 86 32
  
131         
 82 36 132 53 61*
    94 31 192 68
  
164         
 87 19 217 83 84*
    84 18 212 84
  
205         
 92 16 317 115 130*
    94 15 408 145
  
256         
 111 15 462 139
    143r 19 349 81
  
320         
 90 12 515 191 154*
    84 12 295 117
  
400         
 65 6 391 201 212*
    56 5 374 223
Positive Control 3-Methylcholanthrene 
5         
 47 22 439 311 398*
    37 19 447 399
    38 18 548 485

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination