Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01482-0008-0000-5
Chemical Name:	4-Amino-2-nitrophenol
CASRN:	119-34-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G61063
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	101	81	31	37
			81	99	101	42
			82	95	90	37
			89	105	100	37
Test Chemical	4-Amino-2-nitrophenol	3.6	118	123	188	53	51
			91	90	135	49	51
		5.15	111	103	162	49	55
			85	94	158	62	55
		10.5	88	55	403	153	138*
			100	54	366	122	138*
		15	69	17	508	245	258*
			55	21	448	271	258*
Positive Control	Ethyl Methane Sulfonate	500	51	39	1010	658	612*
			60	40	1075	599
			62	42	1069	578

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	99	93	39	35
			82	129	93	38
			90	71	78	29

Test Chemical	4-Amino-2-nitrophenol	7.68	84	87	196	78	62*
			91	97	125	46	62*
		9.6	52	41	228	147	139*
			70	47	275	131	139*
		12	67	50	285	142	142*
			71	42	305	143	142*
		15	53	18	430	271	282*
			56	19	489	294	282*
		18.8	36	6	500	465
Positive Control	Ethyl Methane Sulfonate	500	28	16	1009	1194	1312*
			22	14	950	1450
			28	20	1071	1290

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	132	192	65	74
			87#	105	316.5	122
			96	108	142	49
			93	55	163	59
Test Chemical	4-Amino-2-nitrophenol	6	88	86	392	149	156*
			87	61	421	162	156*
		7.1	79	52	455	193	219*
			74	54	546	246	219*
		8.4	82	60	487	198	204*
			81	58	510	209	204*
		9.8	65	39	503	257
		11.6	60	29	531	297	288*
			69	33	577	279	288*
		13.6	61	24	559	305	319*
			51	18	505	332	319*
		16	44	15	546	418	364*
			55	18	507	310	364*
Positive Control	3-Methylcholanthrene	5	66	61	612	311	321*
			67	58	641	318
			57	47	576	335

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	42r	68	78	62	44
			59	95	86	49
			70	112	75	36
			59	93	85	48
Test Chemical	4-Amino-2-nitrophenol	6	58	81	381	220	202*
			80	106	443	184	202*
		7.1	54	77	350	215	177*
			64	86	269	139	177*
		8.4	52	71	295	190	146*
			62	83	189	102	146*
		9.8	54	68	429	266	230*
			75	93	437	194	230*
		11.6	62	71	445	240	193*
			54	55	238	146	193*
		13.6	64#	60	610.5	320	299*
			50	46	417	277	299*
		16	45	33	468	348	386*
			39	33	498	424	386*
Positive Control	3-Methylcholanthrene	5	54	66	440	270	261*
			64	86	441	229
			43	56	371	285

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination