Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01482-0009-0000-6
Chemical Name:	4-Amino-2-nitrophenol
CASRN:	119-34-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G81743
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	88	74	32	26
			78	97	45	19
			85	115	66	26
			89	100	74	28
Test Chemical	4-Amino-2-nitrophenol	2.5	94	90	86	31	32
			81	82	79	32	32
		5	73	77	96	44	36
			72	83	60	28	36
		10	59	51	84	47	42
			81	71	89	37	42
		15	89	54	195	73	86*
			81	53	240	99	86*
		20	56	22	277	166	151*
			61	38	248	136	151*
		30	40	8	323	270	223*
			63	16	335	176	223*
Positive Control	Ethyl Methane Sulfonate	500	46	35	713	520	544*
Positive Control			52	32	895	568	544*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	101	98	35	37
			83	93	87	35
			102	109	100	33
			77	97	102	44
Test Chemical	4-Amino-2-nitrophenol	2.5	89	78	78	29	25
			94#	90	60	21	25
		5	81	66	101	42	35
			98	97	84	29	35
		10	63	28	283	151	136*
			82	33	301	122	136*
		15	56	10	352	210	222*
			54	10	380	234	222*
		20	53	5	471	298	341*
			38	4	432	384	341*
		30
			40	2	589	493
		40

Positive Control	Ethyl Methane Sulfonate	500	33	17	672	679	645*
			47	25	923	662
			42	25	742	594

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	89	121	52	53
			73	111	93	43
			66	90	117	60
			79	110	141	59
Test Chemical	4-Amino-2-nitrophenol	2.5	67	41	172	85	84*
			68	54	170	83	84*
		3.75	76	69	160	71	73
			79	70	180	76	73
		5	74	76	202	91	89*
			79	69	206	87	89*
		7.5	75	51	324	143	138*
			71	55	281	133	138*
		10	82	40	324	133	133*
			80	45	320	134	133*
		15	62	25	328	177	144*
			74	37	244	110	144*
Positive Control	3-Methylcholanthrene	5	65	42	582	297	310*
Positive Control			62	49	599	323	310*

Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	93	161	65	54
			82	109	132	54
			98	114	104	35
			89	84	164	62
Test Chemical	4-Amino-2-nitrophenol	0.83	99	63	111	37	46
			100	51	164	55	46
		1.65	89	56	146	54	46
			89	54	102	38	46
		3.3	90	38	195	72	70
			83	33	168	68	70
		6.7
			74	12	352	159
		13.3

Positive Control	3-Methylcholanthrene	5	62	29	485	263	283*
			67#	27	590	292
			72	20	632	294

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	105	68	35	38
			63	85	82	44
			77	114	77	33
			78	96	91	39
Test Chemical	4-Amino-2-nitrophenol	5	37	18	116	104	97*
			31	15	89	97
			31	16	84	92
		7.5	54	17	161	100	112*
			59	15	188	107
			27#	11	105	130
		10	36	13	141	131	136*
			36	9	169	159
			39	9	138	119
		12.5	26#	11	145	186	163*
			42	12	164	129
			29	8	150	175
		15	50	15	184	124	117*
			34	19	119	117
			38	17	126	110
		20	38	7	286	250	151*
			38	14	129	112
			44	18	122	92
Positive Control	3-Methylcholanthrene	5	23	5	176	255	279*
			12	3	95	260
			19	4	178	321

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination