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Study Information
Study Attribute Value
CEBS Accession Number: 002-01488-0004-0000-7
Chemical Name: 4-Chloro-2-nitroaniline
CASRN: 89-63-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 971790
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 109 104 129 40 34
    106 105 98 31
    102 106 88 29
    85 84 89 35
Test Chemical 4-Chloro-2-nitroaniline 
2.5       
 100 96 141 47 40
    82 95 88 36
    108 92 123 38
  
5         
 79 75 79 33 33
    97 77 87 30
    83 96 89 36
  
7.5       
 110 84 95 29 33
    97 83 113 39
    88 71 85 32
  
10         
 103 106 117 38 35
    90 75 77 29
    87 80 97 37
  
15         
 104 75 115 37 34
    81 74 82 34
    71 71 67 31
  
20         
 111 87 109 33 37
    99 83 123 41
    84 65 93 37
Positive Control Methyl Methane Sulfonate 
5         
 62 46 570 309 307*
    87 52 727 279
    69 58 687 334

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 87 97 121 46 44
    72 104 98 46
    98# 103 115 39
    98 96 136 46
Test Chemical 4-Chloro-2-nitroaniline 
12.5       
 93 80 87 31 31
    99 67 98 33
    111 82 101 30
  
25         
 78 69 57 25 36
    86 57 105 41
    78 55 98 42
  
37.5       
 64 51 99 52 47
    95 60 118 41
    94 67 134 48
  
50         
 81# 45 95 39 54
    64 48 95 50
    51 42 110 72
  
75         
 75 18 140 63 45
    80 38 100 42
    99 42 92 31
  
100         
 72 19 177 82 92*
    79# 17 214 90
    77 14 239 103
Positive Control Methyl Methane Sulfonate 
5         
 73 87 470 214 207*
    102 66 631 207
    74# 58 441 199

Nonactivation Trial: 3 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 81# 80 60 25 22
    89# 117 44 17
    89 102 68 25
Test Chemical 4-Chloro-2-nitroaniline 
30         
 87# 67 49 19
  
40         
 69 48 46 22 19
    67 31 31 15
  
50         
 61 38 71 39 32
    71 41 52 25
  
60         
 64# 36 75 39 34
    96 23 82 29
  
80         
 81# 29 85 35 30
    72 34 59 27
    75 35 63 28
  
100         
 22 3 159 239 136*
    83 22 83 33
Positive Control Methyl Methane Sulfonate 
5         
 66# 42 411 207 221*
    60# 37 425 235

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 93 95 78 28 26
    87# 94 70 27
    106 110 78 25
    87 101 63 24
Test Chemical 4-Chloro-2-nitroaniline 
60         
 70 47 54 26 26
    81 61 69 28
    97 67 66 23
  
80         
 93 68 69 25 32
    77 41 74 32
    76 31 87 38
  
90         
 65# 35 92 47 47*
    83 34 90 36
    72 20 128 59
  
100         
 64 7 293 153 119*
    101 58 85 28
    58 6 301 174
Positive Control Methyl Methane Sulfonate 
5         
 38 38 418 370 340*
    51# 27 491 324
    58 34 562 325

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 67# 83 109 54 54
    99 120 144 49
    83 103 127 51
    68# 95 128 63
Test Chemical 4-Chloro-2-nitroaniline 
 0.125     
 95 105 87 30 33
    82 110 65 26
    82 81 107 44
  
 0.25      
 69 96 87 42 53
    45# 36 91 68
    77# 87 111 48
  
 0.5       
 56 98 77 46 40
    74 89 86 39
    88 49 93 35
  
1         
 66 44 150 76 76
    46 38 124 89
    69 73 130 62
  
2         
 61 17 397 217 247*
    50 9 372 249
    40 14 329 275
  
3         
 39 10 471 408 425*
    33 6 429 436
    31 7 407 433
Positive Control 3-Methylcholanthrene 
2.5       
 621*
    39 12 733 635
    38 11 696 608

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 89# 114 58 22 23
    72 108 60 28
    83 88 54 22
    86 90 52 20
Test Chemical 4-Chloro-2-nitroaniline 
 0.25      
 65 73 53 27 33
    58 79 65 37
    50 76 52 35
  
 0.5       
 70 78 50 24 24
    56 72 41 25
  
1         
 84 58 103 41 45*
    72 58 107 50
    70 64 92 44
  
2         
 61 23 181 99 106*
    54# 34 145 90
    54 26 208 128
  
3         
 66 8 433 218 201*
    58 14 350 199
    52 11 287 185
  
4         
 56 8 417 248 288*
    38 6 443 390
    65 7 435 225
Positive Control 3-Methylcholanthrene 
2.5       
 70 60 659 315 314*
    72 48 575 266
    51# 42 557 362

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination