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Study Information
Study Attribute Value
CEBS Accession Number: 002-01492-0001-0000-9
Chemical Name: 4-Chloro-m-phenylenediamine
CASRN: 5131-60-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 618847_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 11/05/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 386 0.368 7.72 26.5 0.000
Test Chemical 4-Chloro-m-phenylenediamine 
46.2       
 50 1037 554 0.534 11.08 26.5 45.323 
139         
 50 1045 700 0.670 14.00 26.5 82.215 
462         
 50 1050 1113 1.060 22.26 26.5 188.342 
1385         
 0 0 0 0.000 0.00 30.0 N/A
Positive Control Mitomycin-C 
 0.003     
 50 1042 546 0.524 10.92 26.5 42.537
Mitomycin-C 
 0.01      
 10 209 193 0.923 19.30 26.5 151.196
Trend: 19.840        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 01/14/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 403 0.386 8.06 26.5 0.000
Test Chemical 4-Chloro-m-phenylenediamine 
31.2       
 50 1049 551 0.525 11.02 26.0 35.943 
187         
 50 1051 924 0.879 18.48 26.0 127.535 
373         
 50 1045 1210 1.158 24.20 26.0 199.674 
620         
 0 0 0 0.000 0.00 30.0 N/A
Positive Control Mitomycin-C 
 0.002     
 50 1049 711 0.678 14.22 26.5 75.418
Mitomycin-C 
 0.01      
 10 210 241 1.148 24.10 26.5 197.014
Trend: 22.393        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 11/05/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 428 0.409 8.56 26.0 0.000
Test Chemical 4-Chloro-m-phenylenediamine 
139         
 50 1039 516 0.497 10.32 26.0 21.373 
462         
 50 1042 574 0.551 11.48 26.0 34.627 
1385         
 50 1041 729 0.700 14.58 26.0 71.145 
4620         
 0 0 0 0.000 0.00 30.0 N/A
Positive Control Cyclophosphamide 
 0.5       
 50 1041 690 0.663 13.80 26.0 61.989
Cyclophosphamide 
2.5       
 10 209 289 1.383 28.90 26.0 237.940
Trend: 9.088        
Probability: 0.000