COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01492-0002-0000-0
Chemical Name: 4-Chloro-m-phenylenediamine
CASRN: 5131-60-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 618847_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Questionable 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 01/09/1986    Harvest Time: 10.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 7 0.035 3.5 0 0.000 0.0 7 0.035 4.0 0 0.000 0.0
Test Chemical: 4-Chloro-m-phenylenediamine 
148         
 200 17 0.085 7.5 2 0.010 1.0 15 0.075 7.0 0 0.000 0.0 
494         
 200 12 0.060 6.0 1 0.005 1.0 11 0.055 6.0 0 0.000 0.0 
1480         
 200 32 0.160 9.5 2 0.010 1.0 20 0.100 9.0 10 0.050 1.0 
2960         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 50 41 0.820 42.0 8 0.160 16.0 23 0.460 28.0 10 0.200 1.0
Mitomycin-C 
5         
 50 67 1.340 60.0 22 0.440 32.0 35 0.700 46.0 10 0.200 1.0
Trend: 2.056 1.072 1.766  
Probability: 0.020 0.142 0.039  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 01/09/1986    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4-Chloro-m-phenylenediamine 
148         
 200 5 0.025 1.0 0 0.000 0.0 5 0.025 1.0 0 0.000 0.0 
494         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1480         
 200 1 0.005 0.5 1 0.005 1.0 0 0.000 0.0 0 0.000 0.0 
2960         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 25 0.500 32.0 10 0.200 18.0 15 0.300 20.0 0 0.000 0.0
Trend: 0.196 1.909 -0.715  
Probability: 0.422 0.028 0.763  


Trial #:2   Activation: No Activation    Date: 01/30/1986    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 2 0.010 1.0 1 0.005 1.0 0 0.000 0.0
Test Chemical: 4-Chloro-m-phenylenediamine 
525         
 200 36 0.180 15.0 18 0.090 8.0 18 0.090 8.0 0 0.000 0.0 
790         
 200 41 0.205 14.5 19 0.095 9.0 22 0.110 9.0 0 0.000 0.0 
1060         
 200 30 0.150 11.0 14 0.070 6.0 16 0.080 7.0 0 0.000 0.0 
1580         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.2       
 100 75 0.750 39.0 30 0.300 24.0 23 0.230 18.0 22 0.220 4.0
Mitomycin-C 
5         
 15 134 8.933 100.0 5 0.333 20.0 9 0.600 20.0 120 8.000 12.0
Trend: 3.011 2.249 2.579  
Probability: 0.001 0.012 0.005