Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01495-0009-0000-0
Chemical Name:	4-Chloro-o-toluidine hydrochloride
CASRN:	3165-93-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	151592
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	108	110	122	38	40
			104#	96	111	36
			96	91	134	47
			108	103	133	41
Test Chemical	4-Chloro-o-toluidine hydrochloride	10	79#	54	96	41	59
			79#	45	123	52
			52#	29	128	83
		20	80#	28	187.5	78	69*
			71	25	143	67
			73#	34	136.5	63
		30	43	23	135	105	63*
			83	46	117	47
			116	65	125	36
		40


		50	85#	22	156	61	88*
			81	13	222	92
			67	10	223	111
		60	78	15	213	92	90*
			61#	12	165	90
			95	12	255	89
Positive Control	Ethyl Methane Sulfonate	250	43#	20	609	478	477*
			49	26	676	465
			54	27	791	488

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	92#	110	244.5	89	88
			85	89	260	102
			89	91	234	88
			82#	110	184	75
Test Chemical	4-Chloro-o-toluidine hydrochloride	40	107	79	275	86	85
			102	63	270	88
			57	57	136	80
		50	93	91	174	62	81
			105#	70	304.5	96
			77	57	197	85
		60	98#	67	72	25	79
			80	51	294	123
			77#	59	207	90
		80	106	30	272	86	102
			108	15	337	104
			86	33	303	118
		100	80	23	282	117	117
			100	11	399	133
			105	19	311	99
		120	68#	5	323	158	159*
			84	15	392	156
			69#	8	336	162
Positive Control	Ethyl Methane Sulfonate	250	58#	57	829.5	479	480*
			69#	65	898.5	434
			62	52	976	526

Nonactivation Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	90#	111	186	69	68
			83	89	188	75
			87	91	189	73
			80#	110	138	57
Test Chemical	4-Chloro-o-toluidine hydrochloride	40	106	80	208	66	64
			101	63	192	63
			56	57	107	64
		50	92	92	124	45	63
			105#	71	234	74
			76	57	159	70
		60	96#	67	189	66	78
			78	51	228	98
			76#	59	159	70
		80	104	30	199	64	77
			108	15	237	73
			84	33	233	93
		100	78	23	189	81	85
			98	11	288	98
			103	19	235	76
		120	67#	5	234	117	117*
			82	15	287	117
			67#	8	236	117
Positive Control	Ethyl Methane Sulfonate	250	57#	57	723	427	427*
			67#	64	786	391
			60	52	835	463

Nonactivation Trial: 4 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	92#	110	87	32	33
			85	89	90	35
			89	91	102	38
			82#	110	67	27
Test Chemical	4-Chloro-o-toluidine hydrochloride	40	57	57	34	20	21
			107	79	74	23
			102	63	65	21
		50	77	57	60	26	25
			105#	70	90	28
			93	91	58	21
		60	77#	59	63	27	30
			98#	67	72	25
			80	51	89	37
		80	86	33	89	35	30
			108	15	105	32
			106	30	75	24
		100	80	23	77	32	31
			100	11	99	33
			105	19	85	27
		120	69#	8	78	38	41
			68#	5	88	43
			84	15	109	44
Positive Control	Ethyl Methane Sulfonate	250	62	52	176	95	101*
			69#	65	139.5	67
			58#	57	246	142

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	77	93	93	40	32
			84	94	83	33
			104	112	74	24
Test Chemical	4-Chloro-o-toluidine hydrochloride	2.5	77#	69	57	25	23
			60	65	45	25
			87	86	52	20
		5	77#	61	79	34	29
			78	44	65	28
			70	65	53	25
		10	74	58	94	42	41
			50	48	60	40
			70	42	85	41
		15	64#	31	120	62	59*
			64#	41	120	63
			69	29	107	51
		20	83	16	143	57	72*
			97#	20	203	70
			61	15	164	89
		30


Positive Control	Ethyl Methane Sulfonate	250	76	41	1072	472	548*
			68	48	1020	501
			49	23	976	671

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	73	105	73	33	31
Vehicle Control			86	95	74	29	31
Test Chemical	4-Chloro-o-toluidine hydrochloride	1.56	104	111	75	24
		3.13	72	53	72	34
		6.25	78	56	66	28	33
			76	44	77	34
			66	46	71	36
		12.5	73	37	73	33	35
			88	49	87	33
			81	35	95	39
		25	71	10	133	62	53*
			105	36	138	44	53*
		50					54*
			103	14	170	55
			58	21	91	52
		100


Positive Control	3-Methylcholanthrene	2.5	100	38	631	210	231*
Positive Control			76	37	573	251	231*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination