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Study Information
Study Attribute Value
CEBS Accession Number: 002-01498-0002-0000-6
Chemical Name: 4-Chloronitrobenzene
CASRN: 100-00-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 565306_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Positive 
No Activation  3  Weakly Positive* 

Trial #:1   Activation: No Activation    Date: 12/08/1981    Harvest Time: 19 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4-Chloronitrobenzene 
500         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
600         
 50 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
700         
 22 1 0.045 5.0 1 0.045 5.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 100 26 0.260 21.0 18 0.180 16.0 8 0.080 7.0 0 0.000 0.0
Trend: 2.214 2.214 0.000  
Probability: 0.013 0.013 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 11/16/1981    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 4-Chloronitrobenzene 
50         
 100 4 0.040 4.0 3 0.030 3.0 0 0.000 0.0 1 0.010 1.0 
167         
 100 5 0.050 5.0 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 
500         
 100 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 1 0.010 1.0 
1670         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5000         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
25         
 100 24 0.240 20.0 14 0.140 12.0 8 0.080 8.0 2 0.020 2.0
Trend: -1.199 -1.477 0.075  
Probability: 0.885 0.930 0.470  


Trial #:2   Activation: No Activation    Date: 11/16/1981    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 4-Chloronitrobenzene 
50         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
167         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1670         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 100 22 0.220 19.0 18 0.180 16.0 4 0.040 4.0 0 0.000 0.0
Trend: -0.424 0.293 -1.352  
Probability: 0.664 0.385 0.912  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 12/18/1981    Harvest Time: 19 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 1.0 2 0.020 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4-Chloronitrobenzene 
600         
 35 6 0.171 17.0 3 0.086 9.0 2 0.057 6.0 1 0.029 1.0 
700         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
800         
 100 38 0.380 31.0 12 0.120 11.0 24 0.240 22.0 2 0.020 2.0 
900         
 100 4 0.040 3.0 1 0.010 1.0 2 0.020 1.0 1 0.010 1.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 236 4.720 88.0 63 1.260 56.0 33 0.660 42.0 140 2.800 28.0
Trend: 2.553 1.189 2.315  
Probability: 0.005 0.117 0.010  


Trial #:3   Activation: No Activation    Date: 11/23/1981    Harvest Time: 10.7 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 4 0.040 3.0 4 0.040 3.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4-Chloronitrobenzene 
700         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
800         
 84 5 0.060 6.0 1 0.012 1.0 4 0.048 5.0 0 0.000 0.0 
900         
 33 8 0.242 24.0 6 0.182 18.0 2 0.061 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 100 16 0.160 16.0 9 0.090 9.0 7 0.070 7.0 0 0.000 0.0
Trend: 3.802 2.445 2.983  
Probability: 0.000 0.007 0.001