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Study Information
Study Attribute Value
CEBS Accession Number: 002-01500-0003-0000-1
Chemical Name: 4-Dimethylaminoazobenzene
CASRN: 60-11-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 258568
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 99 130 56 48
    80 112 109 46
    76# 91 121 53
    82# 98 93 38
Test Chemical 4-Dimethylaminoazobenzene 
20         
 84 92 93 37 38
    75 83 89 39
  
25         
 85 97 165 65 58
    88 108 134 51
  
31         
 76 84 97 42 65
    64 69 170 88
  
38         
 66 76 148 75 90*
    82 87 256 104
  
48         
 73 84 115 52 72
    62 61 169 91
  
60         
 73 84 100 46 57
    63 73 129 68
Positive Control Ethyl Methane Sulfonate 
500         
 42 39 758 597 569*
    37 36 713 642
    53 52 747 468

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 62 91 57 31 28
    68 93 53 26
    64 90 66 35
    92 125 61 22
Test Chemical 4-Dimethylaminoazobenzene 
47         
 58 73 65 38 41
    65 81 74 38
    48 66 69 48
  
52         
 58# 74 30 17 29
    67 87 68 34
    68 85 73 36
  
58         
 64 81 48 25 28
    69 86 69 34
    67 80 48 24
  
65         
 68 81 67 33 29
    61 71 50 27
    59 78 48 27
  
72         
 64 71 39 20 22
    62 75 49 26
    70 79 43 20
  
80         
 70 79 65 31 27
    67 70 42 21
    58 65 50 29
Positive Control Ethyl Methane Sulfonate 
500         
 48 53 660 463 452*
    39 39 601 514
    47 53 535 378

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 116 144 55 41
    78 98 84 36
    74 89 91 41
    72 97 73 34
Test Chemical 4-Dimethylaminoazobenzene 
8         
 75 98 98 44 51
    74 106 130 58
  
10         
 70 86 147 70 66*
    74 88 136 62
  
13         
 79 81 149 63 64
    80 84 155 65
  
16         
 66 40 178 89 83*
    64 39 147 77
  
20         
 58 18 120 69 95*
    44 15 159 120
  
25         
 34 6 131 129 132*
    33 6 134 135
Positive Control 3-Methylcholanthrene 
5         
 61 76 361 197 215*
    61 73 365 198
    60 68 445 249

Induced S9 Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 104 70 32 35
    56 89 71 43
    85 107 77 30
    124r 149 103 28
Test Chemical 4-Dimethylaminoazobenzene 
9         
 67 84 86 43 52
    67 88 121 61
  
11         
 79 85 63 27 27
    76 81 61 27
  
14         
 69 50 79 38 51
    50 43 95 63
  
18         
 61# 17 25.5 14 10
    60 24 12 7
  
22         
 22 2 9 14
  
28         
   
Positive Control 3-Methylcholanthrene 
6         
 49 35 331 225 244*
    40 32 122 103
    38 29 455 404

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 94 91 128 46 38
    118 114 137 39
    108 105 95 29
    98 89 115 39
Test Chemical 4-Dimethylaminoazobenzene 
16         
 83 36 158 63 60*
    77 30 182 79
    100# 42 111 37
  
18         
 72 23 131 61 69*
    76 22 175 77
    84 31 173 69
  
20         
 73 18 164 75 94*
    62 17 209 112
  
22         
 79 14 158 67 66*
    86 14 204 79
    92 20 147 53
  
24         
 65 7 140 71 85*
    64 8 195 102
    71 9 173 81
  
27         
 40 3 124 102 120*
    53 4 224 141
    45 3 157 117
  
30         
   
   
Positive Control 3-Methylcholanthrene 
5         
 59 39 585 331 282*
    59 41 504 286
    76 62 526 231

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination