Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01500-0003-0000-1
Chemical Name:	4-Dimethylaminoazobenzene
CASRN:	60-11-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	258568
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	99	130	56	48
			80	112	109	46
			76#	91	121	53
			82#	98	93	38
Test Chemical	4-Dimethylaminoazobenzene	20	84	92	93	37	38
			75	83	89	39	38
		25	85	97	165	65	58
			88	108	134	51	58
		31	76	84	97	42	65
			64	69	170	88	65
		38	66	76	148	75	90*
			82	87	256	104	90*
		48	73	84	115	52	72
			62	61	169	91	72
		60	73	84	100	46	57
			63	73	129	68	57
Positive Control	Ethyl Methane Sulfonate	500	42	39	758	597	569*
			37	36	713	642
			53	52	747	468

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	62	91	57	31	28
			68	93	53	26
			64	90	66	35
			92	125	61	22
Test Chemical	4-Dimethylaminoazobenzene	47	58	73	65	38	41
			65	81	74	38
			48	66	69	48
		52	58#	74	30	17	29
			67	87	68	34
			68	85	73	36
		58	64	81	48	25	28
			69	86	69	34
			67	80	48	24
		65	68	81	67	33	29
			61	71	50	27
			59	78	48	27
		72	64	71	39	20	22
			62	75	49	26
			70	79	43	20
		80	70	79	65	31	27
			67	70	42	21
			58	65	50	29
Positive Control	Ethyl Methane Sulfonate	500	48	53	660	463	452*
			39	39	601	514
			47	53	535	378

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	116	144	55	41
			78	98	84	36
			74	89	91	41
			72	97	73	34
Test Chemical	4-Dimethylaminoazobenzene	8	75	98	98	44	51
			74	106	130	58	51
		10	70	86	147	70	66*
			74	88	136	62	66*
		13	79	81	149	63	64
			80	84	155	65	64
		16	66	40	178	89	83*
			64	39	147	77	83*
		20	58	18	120	69	95*
			44	15	159	120	95*
		25	34	6	131	129	132*
			33	6	134	135	132*
Positive Control	3-Methylcholanthrene	5	61	76	361	197	215*
			61	73	365	198
			60	68	445	249

Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	104	70	32	35
			56	89	71	43
			85	107	77	30
			124r	149	103	28
Test Chemical	4-Dimethylaminoazobenzene	9	67	84	86	43	52
			67	88	121	61	52
		11	79	85	63	27	27
			76	81	61	27	27
		14	69	50	79	38	51
			50	43	95	63	51
		18	61#	17	25.5	14	10
			60	24	12	7	10
		22	22	2	9	14
		28

Positive Control	3-Methylcholanthrene	6	49	35	331	225	244*
			40	32	122	103
			38	29	455	404

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	91	128	46	38
			118	114	137	39
			108	105	95	29
			98	89	115	39
Test Chemical	4-Dimethylaminoazobenzene	16	83	36	158	63	60*
			77	30	182	79
			100#	42	111	37
		18	72	23	131	61	69*
			76	22	175	77
			84	31	173	69
		20	73	18	164	75	94*
			62	17	209	112	94*
		22	79	14	158	67	66*
			86	14	204	79
			92	20	147	53
		24	65	7	140	71	85*
			64	8	195	102
			71	9	173	81
		27	40	3	124	102	120*
			53	4	224	141
			45	3	157	117
		30


Positive Control	3-Methylcholanthrene	5	59	39	585	331	282*
			59	41	504	286
			76	62	526	231

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination