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Study Information
Study Attribute Value
CEBS Accession Number: 002-01500-0004-0000-2
Chemical Name: 4-Dimethylaminoazobenzene
CASRN: 60-11-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 451770
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 99 72 27 30
    98 90 109 37
    97 108 92 32
    108 104 82 25
Test Chemical 4-Dimethylaminoazobenzene 
1.56      
 76 67 51 22 24
    93 78 71 25
  
3.13      
 100 94 89 30 27
    97 97 69 24
  
6.25      
 102 89 97 32 27
    75 76 50 22
  
12.5       
 87 85 110 42 33
    84 69 58 23
  
25         
 94 71 80 28 34
    89 74 105 40
  
50         
 91 60 88 32 37
    63 72 79 42
Positive Control Ethyl Methane Sulfonate 
500         
 31 16 765 827 860*
    32 12 857 893

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 93 78 30 35
    85 130 54 21
    55 82 88 53
    78 95 85 37
Test Chemical 4-Dimethylaminoazobenzene 
10         
 106 250 46 14 29
    68 113 86 42
    91 116 83 30
  
20         
 68 68 76 38 38
    59 65 86 48
    107 130 88 27
  
30         
 94# 59 69 24 29
    98 73 86 29
    55 41 55 34
  
40         
 59 47 94 53 46
    70 50 86 41
    63 52 82 43
  
50         
 53 43 108 68 54*
    62 35 94 51
    63 52 82 44
  
60         
 67# 25 72 36 36
    94 53 115 41
    60 23 55 30
Positive Control Ethyl Methane Sulfonate 
250         
 6r 1 106 642 490*
    13# 6 225 570
    22 13 265 411

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 63 90 44 23 26
    69 110 60 29
Test Chemical 4-Dimethylaminoazobenzene 
20         
 67# 80 49 25 21
    69 104 36 17
  
30         
 72 79 69 32 30
    59 67 66 37
    59# 71 37.5 21
  
40         
 66 79 45 23 29
    62 78 56 30
    59# 74 60 34
  
50         
 52 60 48 31 27
    66 78 38 19
    66 86 59 30
  
60         
 56# 63 57 34 26
    53 62 38 24
    63 71 38 20
  
80         
 55# 59 54 33 31
    57 45 51 30
Positive Control Ethyl Methane Sulfonate 
250         
 44 48 690 521 568*
    42 33 768 614
    46 48 789 570

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 94 115 52 44
    100 99 137 46
    101 92 133 44
    104 115 106 34
Test Chemical 4-Dimethylaminoazobenzene 
 0.39      
 88 99 139 53 50
    87 105 121 46
  
 0.78      
 103 89 187 60 62
    78# 87 150 64
  
1.56      
 64 68 138 72 64
    85 87 144 57
  
3.13      
 104 66 177 57 65
    91 55 201 74
  
6.25      
 71 18 235 110 91*
    102 27 219 72
  
12.5       
 99 12 233 79 122*
    56 4 279 165
  
25         
   
Positive Control 3-Methylcholanthrene 
5         
 26 4 315 412 380*
    30 4 280 309
    25 5 315 420

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 107 67 23 20
    85 98 60 23
    97 90 50 17
    86 106 47 18
Test Chemical 4-Dimethylaminoazobenzene 
1.56      
 78# 58 55.5 24 26
    61 55 53 29
    86 71 64 25
  
3.13      
 65 43 70 36 28
    73 51 46 21
  
6.25      
 69 19 96 46 45*
    62 17 82 44
  
10         
 68# 11 118 58 62*
    68 11 156 76
    73 18 110 50
  
12.5       
 85 9 233 92 89*
    78 11 175 75
    48# 7 145.5 101
  
15         
 72 8 179 82 81*
    67 7 161 80
Positive Control 3-Methylcholanthrene 
2.5       
 72 61 376 175 168*
    85 62 398 157
    89 53 459 172

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination