Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01502-0011-0000-2
Chemical Name:	4-Hexylresorcinol
CASRN:	136-77-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	356349
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	53	74	84	53	44
			80	108	96	40
			44r	63	48	37
			75	117	87	39
Test Chemical	4-Hexylresorcinol	1.25	76	86	56	25	26
			71	128	45	21
			57	92	53	31
		2.5	57	70	69	40	30
			82	95	66	27
			95	129	66	23
		5	65	105	73	38	30
			69	124	47	23	30
		10	78	104	44	19	27
			57	82	53	31
			65	117	60	31
		15	71#	26	76	36	36
			92	51	72	26
			64	33	91	47
		20	81	105	64	27


		30


Positive Control	Methyl Methane Sulfonate	5	91	73	520	191	236*
			54	86	365	227
			50	79	436	289

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	105	158	118	37	41
			72#	81	78	36
			75	87	96	43
			74	73	102	46
Test Chemical	4-Hexylresorcinol	2.5	90	132	87	32	29
			100	113	75	25
			97	115	87	30
		5	88	121	65	25	33
			70	138	77	37
			105	133	124	39
		7.5	90	126	90	33	32
			80	117	71	30	32
		10	82	139	91	37	40
			91	116	129	47
			90#	103	99	37
		15	94	67	94	34	37
			87	110	80	31
			83	70	119	48
		20	81	48	107	44	57

			79	14	165	70
		25


Positive Control	Methyl Methane Sulfonate	5	76	66	626	275	288*
			57	52	557	324
			82#	78	657	266

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	105	89	277	88	92
			106	102	273	86
			97	110	256	88
			95	99	306	107
Test Chemical	4-Hexylresorcinol	2.5	70	59	156	74	78
			63	67	144	76
			51#	62	128	83
		5	56	49	273	162	109
			66	47	156	79
			70	48	185	88
		10	82	38	459	186	161*
			82	31	412	169
			69	30	263	128
		15	67	47	314	155	149*
			73	34	297	137
			73	28	334	154
		20	96	21	398	138	129
			74	25	315	141
			102	31	327	107
		30	74	20	247	112	151*
			95	17	561	196
			83	18	363	146
Positive Control	3-Methylcholanthrene	2.5	105	103	538	171	172*
			100	108	448	150
			95	76	557	196

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	79	103	186	78	91
			93	102	279	101
			112	84	325	96
			104	111	276	88
Test Chemical	4-Hexylresorcinol	2.5	74	82	199	90	86
			77	77	206	89
			64	67	149	78
		5	74	61	336	152	145*
			89	73	336	126
			92	83	433	156
		10	74	54	297	135	147*
			72#	48	300	139
			72	50	360	167
		15	87	32	447	171	145*
			84	47	313	124
			104	37	435	139
		20	74	40	517	232	187*
			76	13	440	193
			92	34	373	135
		30	63	6	557	294	201*
			94	14	376	133
			56	9	295	176
		40


Positive Control	3-Methylcholanthrene	2.5	86	102	410	159	225*
			95	108	499	176
			71	33	725	340

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination