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Study Information
Study Attribute Value
CEBS Accession Number: 002-01504-0001-0000-3
Chemical Name: 4-Hydroxypyrazolo[3,4-d]pyrimidine
CASRN: 315-30-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: A78568_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 06/12/1995    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: 4-Hydroxypyrazolo[3,4-d]pyrimidine 
2.5       
 200 7 0.035 3.5 4 0.020 2.0 3 0.015 1.5 0 0.000 0.0 
5         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0 
7.5       
 200 10 0.050 4.5 5 0.025 2.5 5 0.025 2.0 0 0.000 0.0 
10         
 200 9 0.045 3.5 2 0.010 1.0 7 0.035 3.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 48 0.240 22.0 21 0.105 10.5 27 0.135 12.5 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 20 0.400 40.0 9 0.180 18.0 11 0.220 22.0 0 0.000 0.0
Trend: 1.246 1.034 1.019  
Probability: 0.106 0.150 0.154  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 06/12/1995    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 8 0.040 4.0 0 0.000 0.0 8 0.040 4.0 0 0.000 0.0
Test Chemical: 4-Hydroxypyrazolo[3,4-d]pyrimidine 
20         
 200 6 0.030 3.0 0 0.000 0.0 6 0.030 3.0 0 0.000 0.0 
30         
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0 
40         
 200 8 0.040 3.5 0 0.000 0.0 8 0.040 3.5 0 0.000 0.0 
50         
 200 26 0.130 12.0 1 0.005 0.5 25 0.125 12.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 22 0.110 10.5 1 0.005 0.5 21 0.105 10.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 31 0.620 48.0 10 0.200 18.0 21 0.420 34.0 0 0.000 0.0
Trend: 3.038 1.289 3.038  
Probability: 0.001 0.099 0.001  


Trial #:2   Activation: No Activation    Date: 06/27/1995    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Test Chemical: 4-Hydroxypyrazolo[3,4-d]pyrimidine 
5         
 200 6 0.030 2.5 2 0.010 1.0 4 0.020 2.0 0 0.000 0.0 
7.5       
 200 11 0.055 5.0 3 0.015 1.5 8 0.040 4.0 0 0.000 0.0 
10         
 200 7 0.035 3.0 1 0.005 0.5 6 0.030 2.5 0 0.000 0.0 
20         
 200 5 0.025 2.5 1 0.005 0.5 4 0.020 2.0 0 0.000 0.0 
30         
 200 10 0.050 5.0 0 0.000 0.0 10 0.050 5.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 38 0.190 18.5 21 0.105 10.5 17 0.085 8.5 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 23 0.460 42.0 5 0.100 10.0 18 0.360 34.0 0 0.000 0.0
Trend: 1.701 -1.043 2.172  
Probability: 0.044 0.852 0.015