Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01504-0001-0000-3 |
Chemical Name: | 4-Hydroxypyrazolo[3,4-d]pyrimidine |
CASRN: | 315-30-0 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | A78568_CA |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Positive |
Induced Rat Liver S9 | 1_S9 | Positive |
No Activation | 2 | Positive |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 |
Test Chemical: | 4-Hydroxypyrazolo[3,4-d]pyrimidine | 2.5 | 200 | 7 | 0.035 | 3.5 | 4 | 0.020 | 2.0 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 |
5 | 200 | 3 | 0.015 | 1.5 | 1 | 0.005 | 0.5 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
7.5 | 200 | 10 | 0.050 | 4.5 | 5 | 0.025 | 2.5 | 5 | 0.025 | 2.0 | 0 | 0.000 | 0.0 | ||
10 | 200 | 9 | 0.045 | 3.5 | 2 | 0.010 | 1.0 | 7 | 0.035 | 3.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.0625 | 200 | 48 | 0.240 | 22.0 | 21 | 0.105 | 10.5 | 27 | 0.135 | 12.5 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 20 | 0.400 | 40.0 | 9 | 0.180 | 18.0 | 11 | 0.220 | 22.0 | 0 | 0.000 | 0.0 | |
Trend: | 1.246 | 1.034 | 1.019 | ||||||||||||
Probability: | 0.106 | 0.150 | 0.154 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 8 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | 8 | 0.040 | 4.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 4-Hydroxypyrazolo[3,4-d]pyrimidine | 20 | 200 | 6 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | 6 | 0.030 | 3.0 | 0 | 0.000 | 0.0 |
30 | 200 | 5 | 0.025 | 2.5 | 0 | 0.000 | 0.0 | 5 | 0.025 | 2.5 | 0 | 0.000 | 0.0 | ||
40 | 200 | 8 | 0.040 | 3.5 | 0 | 0.000 | 0.0 | 8 | 0.040 | 3.5 | 0 | 0.000 | 0.0 | ||
50 | 200 | 26 | 0.130 | 12.0 | 1 | 0.005 | 0.5 | 25 | 0.125 | 12.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 2.5 | 200 | 22 | 0.110 | 10.5 | 1 | 0.005 | 0.5 | 21 | 0.105 | 10.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 7.5 | 50 | 31 | 0.620 | 48.0 | 10 | 0.200 | 18.0 | 21 | 0.420 | 34.0 | 0 | 0.000 | 0.0 | |
Trend: | 3.038 | 1.289 | 3.038 | ||||||||||||
Probability: | 0.001 | 0.099 | 0.001 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 2 | 0.010 | 1.0 | 1 | 0.005 | 0.5 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 |
Test Chemical: | 4-Hydroxypyrazolo[3,4-d]pyrimidine | 5 | 200 | 6 | 0.030 | 2.5 | 2 | 0.010 | 1.0 | 4 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
7.5 | 200 | 11 | 0.055 | 5.0 | 3 | 0.015 | 1.5 | 8 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | ||
10 | 200 | 7 | 0.035 | 3.0 | 1 | 0.005 | 0.5 | 6 | 0.030 | 2.5 | 0 | 0.000 | 0.0 | ||
20 | 200 | 5 | 0.025 | 2.5 | 1 | 0.005 | 0.5 | 4 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
30 | 200 | 10 | 0.050 | 5.0 | 0 | 0.000 | 0.0 | 10 | 0.050 | 5.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.0625 | 200 | 38 | 0.190 | 18.5 | 21 | 0.105 | 10.5 | 17 | 0.085 | 8.5 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 23 | 0.460 | 42.0 | 5 | 0.100 | 10.0 | 18 | 0.360 | 34.0 | 0 | 0.000 | 0.0 | |
Trend: | 1.701 | -1.043 | 2.172 | ||||||||||||
Probability: | 0.044 | 0.852 | 0.015 |