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Study Information
Study Attribute Value
CEBS Accession Number: 002-01514-0001-0000-4
Chemical Name: 4-Nitro-1,3-benzenediamine
CASRN: 5131-58-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 795909_SCE
Study Result: Weakly Positive

Study Result: Weakly Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 01/21/1986    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 493 0.473 9.86 26.0 0.000
Test Chemical 4-Nitro-1,3-benzenediamine 
1.6       
 50 1049 461 0.439 9.22 26.0 -7.115 
5         
 50 1051 402 0.382 8.04 26.0 -19.157 
16         
 50 1050 470 0.448 9.40 26.0 -5.392 
50         
 50 1046 543 0.519 10.86 26.0 9.721 
160         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1043 632 0.606 12.64 26.0 28.072
Mitomycin-C 
 0.005     
 10 211 346 1.640 34.60 26.0 246.589
Trend: 1.530        
Probability: 0.063        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/21/1986    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 384 0.366 7.68 26.0 0.000
Test Chemical 4-Nitro-1,3-benzenediamine 
1.6       
 50 1043 369 0.354 7.38 26.0 -3.353 
5         
 50 1044 467 0.447 9.34 26.0 22.197 
16         
 50 1047 432 0.413 8.64 26.0 12.715 
50         
 24 503 220 0.437 9.17 26.0 19.481 
160         
 0 0 0 0.000 0.00 26.0 N/A 
500         
 50 1045 441 0.422 8.82 26.0 15.283
Positive Control Cyclophosphamide 
 0.15      
 50 1052 598 0.568 11.96 26.0 55.285
Cyclophosphamide 
 0.6       
 10 210 212 1.010 21.20 26.0 175.779
Trend: 2.582        
Probability: 0.005        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 02/03/1988    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 465 0.445 9.30 26.0 0.000
Test Chemical 4-Nitro-1,3-benzenediamine 
16         
 50 1045 476 0.456 9.52 26.0 2.366 
50         
 50 1048 443 0.423 8.86 26.0 -5.004 
160         
 50 1039 475 0.457 9.50 26.0 2.740 
500         
 50 1047 565 0.540 11.30 26.0 21.273
Positive Control Cyclophosphamide 
 0.15      
 50 1040 638 0.613 12.76 26.0 37.864
Cyclophosphamide 
 0.6       
 10 209 181 0.866 18.10 26.0 94.624
Trend: 2.872        
Probability: 0.002