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Study Information
Study Attribute Value
CEBS Accession Number: 002-01514-0002-0000-5
Chemical Name: 4-Nitro-1,3-benzenediamine
CASRN: 5131-58-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 795909_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive* 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 03/19/1986    Harvest Time: 13 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: 4-Nitro-1,3-benzenediamine 
300         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0 
1000         
 200 17 0.085 7.0 0 0.000 0.0 17 0.085 7.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 200 57 0.285 22.0 16 0.080 7.5 41 0.205 16.5 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 22 0.440 20.0 4 0.080 6.0 8 0.160 12.0 10 0.200 1.0
Trend: 3.758 0.000 3.758  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/22/1986    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0
Test Chemical: 4-Nitro-1,3-benzenediamine 
1600         
 200 193 0.965 72.5 24 0.120 10.0 158 0.790 67.5 11 0.055 2.0 
3000         
 200 232 1.160 73.5 36 0.180 12.0 156 0.780 66.0 40 0.200 4.0 
5000         
 200 130 0.650 59.5 4 0.020 2.0 126 0.630 58.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
5         
 200 39 0.195 17.5 6 0.030 3.0 33 0.165 15.5 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 20 0.400 32.0 2 0.040 4.0 18 0.360 30.0 0 0.000 0.0
Trend: 11.140 1.217 10.740  
Probability: 0.000 0.112 0.000  


Trial #:2   Activation: No Activation    Date: 04/22/1986    Harvest Time: 17.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 4-Nitro-1,3-benzenediamine 
300         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
500         
 200 10 0.050 4.5 0 0.000 0.0 10 0.050 4.5 0 0.000 0.0 
1000         
 200 30 0.150 12.0 2 0.010 1.0 28 0.140 11.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 59 0.295 25.0 25 0.125 11.5 34 0.170 15.5 0 0.000 0.0
Mitomycin-C 
 0.125     
 200 134 0.670 51.5 60 0.300 26.0 74 0.370 32.0 0 0.000 0.0
Trend: 6.185 1.974 5.865  
Probability: 0.000 0.024 0.000