Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01515-0002-0000-6 |
Chemical Name: | 4-Nitro-o-phenylenediamine |
CASRN: | 99-56-9 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 549927_CA |
Study Result: | Negative |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Negative |
Induced Rat Liver S9 | 1_S9 | Negative |
No Activation | 2 | Negative |
Induced Rat Liver S9 | 2_S9 | Negative |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | 4-Nitro-o-phenylenediamine | 160 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
500 | 100 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
1600 | 100 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | ||
5000 | 100 | 6 | 0.060 | 5.0 | 1 | 0.010 | 1.0 | 4 | 0.040 | 3.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.25 | 100 | 25 | 0.250 | 23.0 | 14 | 0.140 | 13.0 | 11 | 0.110 | 11.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 1 | 50 | 27 | 0.540 | 36.0 | 13 | 0.260 | 24.0 | 13 | 0.260 | 18.0 | 1 | 0.020 | 1.0 | |
Trend: | 1.047 | -0.955 | 1.511 | ||||||||||||
Probability: | 0.147 | 0.830 | 0.065 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | 4-Nitro-o-phenylenediamine | 160 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
500 | 100 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | ||
1600 | 100 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
5000 | 100 | 4 | 0.040 | 4.0 | 1 | 0.010 | 1.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 15 | 100 | 38 | 0.380 | 32.0 | 20 | 0.200 | 19.0 | 18 | 0.180 | 17.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 50 | 50 | 51 | 1.020 | 62.0 | 28 | 0.560 | 38.0 | 23 | 0.460 | 40.0 | 0 | 0.000 | 0.0 | |
Trend: | 1.191 | 0.412 | 1.127 | ||||||||||||
Probability: | 0.117 | 0.340 | 0.130 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | 4-Nitro-o-phenylenediamine | 2000 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
3000 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
4000 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
5000 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 1 | 100 | 103 | 1.030 | 57.0 | 69 | 0.690 | 49.0 | 24 | 0.240 | 20.0 | 10 | 0.100 | 1.0 |
Trend: | 0.000 | 0.000 | 0.385 | ||||||||||||
Probability: | 0.500 | 0.000 | 0.350 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | 4-Nitro-o-phenylenediamine | 2000 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
3000 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
4000 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
5000 | 100 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 15 | 100 | 60 | 0.600 | 43.0 | 34 | 0.340 | 26.0 | 26 | 0.260 | 21.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 50 | 50 | 81 | 1.620 | 74.0 | 43 | 0.860 | 58.0 | 17 | 0.340 | 28.0 | 21 | 0.420 | 3.0 | |
Trend: | 0.791 | 1.048 | 0.134 | ||||||||||||
Probability: | 0.215 | 0.147 | 0.447 |