Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01515-0011-0000-6
Chemical Name:	4-Nitro-o-phenylenediamine
CASRN:	99-56-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G19468
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	107	130	48	44
Vehicle Control			86	93	104	40	44
Test Chemical	4-Nitro-o-phenylenediamine	32	100#	108	132	44	47
			86	85	129	50	47
		64	97	93	133	46	43
			97	86	118	40	43
		128	71	34	146	69	118*
			31	19	157	167	118*
		256	90	25	185	69	68
			80	18	162	68	68
		512

Positive Control	Ethyl Methane Sulfonate	500	53	43	593	371	361*
Positive Control			49	37	512	351	361*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	88	97	37	43
Vehicle Control			109	112	157	48	43
Test Chemical	4-Nitro-o-phenylenediamine	64	100	57	157	53	59
			79	46	154	65	59
		128	85	41	192	75	71*
			106	34	210	66	71*
		256	78	15	215	91	99*
			67	13	214	107	99*
		512	43	4	204	158	155*
			45	2	206	152	155*
		640	50#	6	202.5	135	128*
			59	8	216	122	128*
		800	43	6	219	168	193*
			56#	7	363	217	193*
		1000	62	6	202	109	121*
			50	7	196	132	121*
Positive Control	Ethyl Methane Sulfonate	500	55	34	841	508	561*
Positive Control			45	28	825	613	561*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	86	119	42	44
			128r	118	189	49
			108	112	153	47
			100	102	128	43
Test Chemical	4-Nitro-o-phenylenediamine	20	108	99	152	47	51
			103	80	163	53
			108	35	168	52
		45	84	76	136	54
			128r	107	177	46
			124r	104	185	50
		101	111	80	209	63	61
			109	65	202	62
			109	78	186	57
		228	78	20	326	140	123*
			101	30	315	104
			96	26	357	124
		512


Positive Control	Ethyl Methane Sulfonate	500	66	46	1320	663	643*
			60	25	1188	656
			73	22	1332	608

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	102	254	134	130
Vehicle Control			68	98	257	126	130
Test Chemical	4-Nitro-o-phenylenediamine	13.1	68	31	311	152
		16.4	48	18	297	206	212*
			46	18	296	217	212*
		20.5	42	16	318	254	214*
			41	12	216	174	214*
		25.6	29	7	226	258	249*
			39	10	279	241	249*
		32	25	5	257	338	299*
			32	6	246	259	299*
		40	26	4	214	269	270*
			28	5	231	272	270*
		50	21	3	232	371
Positive Control	3-Methylcholanthrene	5	24	12	304	425	460*
Positive Control			21	11	317	495	460*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89	104	184	69	55
			89	100	137	52
			79	94	117	49
			83	103	122	49
Test Chemical	4-Nitro-o-phenylenediamine	13.1	69	21	220	106	108*
			68	24	227	111	108*
		16.4	51	16	212	139	129*
			69	21	245	119	129*
		20.5	56	14	274	165	183*
			50	13	302	202	183*
		25.6	48	8	289	203	214*
			42	10	285	224	214*
		32	43	8	272	211	215*
			47	7	310	219	215*
		40	37	5	297	271	261*
			40	6	301	251	261*
Positive Control	3-Methylcholanthrene	5	43	28	459	354	242*
			66	44	372	188
			74	47	406	182

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination