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Study Information
Study Attribute Value
CEBS Accession Number: 002-01516-0001-0000-6
Chemical Name: 4-Nitroanthranilic acid
CASRN: 619-17-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 235278_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 07/17/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 461 0.441 9.22 27.0 0.000
Test Chemical 4-Nitroanthranilic acid 
50         
 50 1047 425 0.406 8.50 27.0 -7.897 
160         
 50 1049 472 0.450 9.44 27.0 2.093 
500         
 50 1044 610 0.584 12.20 27.0 32.575 
1600         
 0 0 0 0.000 0.00 27.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1047 719 0.687 14.38 27.0 55.816
Trend: 4.989        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 08/21/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 483 0.460 9.66 26.0 0.000
Test Chemical 4-Nitroanthranilic acid 
1000         
 3 62 42 0.677 14.00 31.0 47.265 
1000         
 50 1048 810 0.773 16.20 36.0 68.022 
1250         
 50 1050 799 0.761 15.98 36.0 65.424 
1500         
 50 1050 845 0.805 16.90 36.0 74.948 
2000         
 7 147 163 1.109 23.29 36.0 141.053
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 609 0.580 12.18 26.5 26.087
Mitomycin-C 
 0.01      
 10 210 482 2.295 48.20 26.0 398.965
Trend: 10.224        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/17/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 392 0.375 7.84 26.0 0.000
Test Chemical 4-Nitroanthranilic acid 
50         
 50 1048 398 0.380 7.96 26.0 1.337 
160         
 50 1045 451 0.432 9.02 26.0 15.161 
500         
 50 1039 450 0.433 9.00 26.0 15.569 
1600         
 50 1048 405 0.386 8.10 26.0 3.119 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1047 708 0.676 14.16 26.0 80.440
Cyclophosphamide 
 0.6       
 10 210 208 0.990 20.80 26.0 164.295
Trend: 1.235        
Probability: 0.108        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/21/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 439 0.418 8.78 26.0 0.000
Test Chemical 4-Nitroanthranilic acid 
1500         
 50 1047 418 0.399 8.36 26.0 -4.602 
2000         
 50 1045 441 0.422 8.82 26.0 0.840 
3000         
 22 461 288 0.625 13.09 44.5 49.280 
4000         
 18 377 235 0.623 13.06 44.5 48.949
Positive Control Cyclophosphamide 
 0.3       
 50 1048 563 0.537 11.26 26.0 28.368
Trend: 6.665        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 02/05/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 458 0.436 9.16 26.0 0.000
Test Chemical 4-Nitroanthranilic acid 
1000         
 50 1043 419 0.402 8.38 26.0 -7.901 
2000         
 50 1043 492 0.472 9.84 26.0 8.145 
3000         
 50 1044 683 0.654 13.66 47.0 49.984 
4000         
 50 1042 866 0.831 17.32 47.0 90.535
Positive Control Cyclophosphamide 
 0.3       
 50 1050 687 0.654 13.74 26.0 50.000
Cyclophosphamide 
2         
 10 211 402 1.905 40.20 26.0 336.785
Trend: 13.481        
Probability: 0.000