Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01516-0010-0000-6
Chemical Name:	4-Nitroanthranilic acid
CASRN:	619-17-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	721150
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	97	107	73	25	24
			86	112	66	26
			89	90	57	21
			78	92	56	24
Test Chemical	4-Nitroanthranilic acid	87.5	89	96	70	26	25
			82	109	58	24	25
		175	85	95	68	27	26
			78	82	60	26	26
		350	79	86	62	26	26
			76	81	58	25	26
		700	81	76	78	32	35
			89	85	101	38	35
		1400	45	7	428	317

		2800

Positive Control	Methyl Methane Sulfonate	20	18	10	123	234	266*
Positive Control			18	11	158	298	266*

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	61	88	110	60	59
			52	94	113	72
			57	104	99	58
			73	114	103	47
Test Chemical	4-Nitroanthranilic acid	600	69	106	150	72	66
			64	96	115	60	66
		800	71	90	138	65	72
			60	79	142	79	72
		1000	63#	65	112.5	60	65
			60	69	125	69	65
		1200	68	35	168	83	97*
			56	28	186	111	97*
		1400

Positive Control	Methyl Methane Sulfonate	15	25	26	159	209	208*
Positive Control			22	23	136	206	208*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	102	77	30	33
			97	101	108	37
			100	95	97	32
			83	102	82	33
Test Chemical	4-Nitroanthranilic acid	1000	60	75	160	90	83*
			54	77	122	76	83*
		1100	47	41	168	120	134*
			52	43	231	148	134*
		1200	51	20	257	170	168*
			57	12	285	167	168*
		1300

Positive Control	3-Methylcholanthrene	2.5	49	25	466	320	318*
Positive Control			45	26	423	316	318*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	100	150	72	50
			78	96	91	39
			81	99	116	48
			92	106	118	43
Test Chemical	4-Nitroanthranilic acid	900	53	63	122	77	77
			54	42	124	77	77
		1000	46	44	141	101	97*
			51	49	140	92	97*
		1100	67	55	189	94	86*
			55	35	128	78	86*
		1200	43	47	91	70	83*
			54	29	155	95	83*
		1300	5r	2	304	1961
			38r	14	170	150
Positive Control	3-Methylcholanthrene	2.5	46	38	447	327	372*
Positive Control			35	22	434	417	372*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	116	121	56	67
			56	77	158	95
			61	101	92	51
			77	107	155	68
Test Chemical	4-Nitroanthranilic acid	800	53	61	66	41	38
			46	51	47	34	38
		900	51	41	62	41	43
			52	58	70	45	43
		1000	52	42	112	71	70
			54	42	110	69	70
		1100	34	22	89	86	76
			34	31	67	66	76
		1200

Positive Control	3-Methylcholanthrene	2.5	23	12	252	368	357*
Positive Control			28	14	285	345	357*

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	129	68	23	29
			76	80	78	34
			74	95	66	30
			75	95	63	28
Test Chemical	4-Nitroanthranilic acid	800	59	70	70	40	42
			77	106	92	40
			83	78	114	46
		900	77	103	104	45	46*
			74	85	95	43
			71	87	105	49
		1000	79	64	138	59	54*
			68	76	66	33
			86	80	178	69
		1100	85	67	116	45	65*
			76	51	183	80
			77	60	158	68
		1200	86	76	105	41	46*
			76	42	100	44
			84	56	133	53
		1300	60	13	106	59	58*
			94	25	147	52
			71	19	131	62
Positive Control	3-Methylcholanthrene	2.5	43	23	362	284	263*
Positive Control			47	28	338	242	263*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination