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Study Information
Study Attribute Value
CEBS Accession Number: 002-01516-0010-0000-6
Chemical Name: 4-Nitroanthranilic acid
CASRN: 619-17-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 721150
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 97 107 73 25 24
    86 112 66 26
    89 90 57 21
    78 92 56 24
Test Chemical 4-Nitroanthranilic acid 
87.5       
 89 96 70 26 25
    82 109 58 24
  
175         
 85 95 68 27 26
    78 82 60 26
  
350         
 79 86 62 26 26
    76 81 58 25
  
700         
 81 76 78 32 35
    89 85 101 38
  
1400         
 45 7 428 317
   
  
2800         
   
Positive Control Methyl Methane Sulfonate 
20         
 18 10 123 234 266*
    18 11 158 298

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 61 88 110 60 59
    52 94 113 72
    57 104 99 58
    73 114 103 47
Test Chemical 4-Nitroanthranilic acid 
600         
 69 106 150 72 66
    64 96 115 60
  
800         
 71 90 138 65 72
    60 79 142 79
  
1000         
 63# 65 112.5 60 65
    60 69 125 69
  
1200         
 68 35 168 83 97*
    56 28 186 111
  
1400         
   
Positive Control Methyl Methane Sulfonate 
15         
 25 26 159 209 208*
    22 23 136 206

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 102 77 30 33
    97 101 108 37
    100 95 97 32
    83 102 82 33
Test Chemical 4-Nitroanthranilic acid 
1000         
 60 75 160 90 83*
    54 77 122 76
  
1100         
 47 41 168 120 134*
    52 43 231 148
  
1200         
 51 20 257 170 168*
    57 12 285 167
  
1300         
   
Positive Control 3-Methylcholanthrene 
2.5       
 49 25 466 320 318*
    45 26 423 316

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 69 100 150 72 50
    78 96 91 39
    81 99 116 48
    92 106 118 43
Test Chemical 4-Nitroanthranilic acid 
900         
 53 63 122 77 77
    54 42 124 77
  
1000         
 46 44 141 101 97*
    51 49 140 92
  
1100         
 67 55 189 94 86*
    55 35 128 78
  
1200         
 43 47 91 70 83*
    54 29 155 95
  
1300         
 5r 2 304 1961
    38r 14 170 150
Positive Control 3-Methylcholanthrene 
2.5       
 46 38 447 327 372*
    35 22 434 417

Induced S9 Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 116 121 56 67
    56 77 158 95
    61 101 92 51
    77 107 155 68
Test Chemical 4-Nitroanthranilic acid 
800         
 53 61 66 41 38
    46 51 47 34
  
900         
 51 41 62 41 43
    52 58 70 45
  
1000         
 52 42 112 71 70
    54 42 110 69
  
1100         
 34 22 89 86 76
    34 31 67 66
  
1200         
   
Positive Control 3-Methylcholanthrene 
2.5       
 23 12 252 368 357*
    28 14 285 345

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 100 129 68 23 29
    76 80 78 34
    74 95 66 30
    75 95 63 28
Test Chemical 4-Nitroanthranilic acid 
800         
 59 70 70 40 42
    77 106 92 40
    83 78 114 46
  
900         
 77 103 104 45 46*
    74 85 95 43
    71 87 105 49
  
1000         
 79 64 138 59 54*
    68 76 66 33
    86 80 178 69
  
1100         
 85 67 116 45 65*
    76 51 183 80
    77 60 158 68
  
1200         
 86 76 105 41 46*
    76 42 100 44
    84 56 133 53
  
1300         
 60 13 106 59 58*
    94 25 147 52
    71 19 131 62
Positive Control 3-Methylcholanthrene 
2.5       
 43 23 362 284 263*
    47 28 338 242

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination