Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01519-0003-0000-1
Chemical Name:	4-Nitroquinoline-N-oxide
CASRN:	56-57-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G23980
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	117	113	64	18	17
Vehicle Control			89	70	44	16	17
Test Chemical	4-Nitroquinoline-N-oxide	0.0195	113	34	141	41	61*
			52	24	127	81	61*
		0.039	75	20	221	99	89*
			74	15	176	79	89*
		0.078	55	28	164	99	110*
			49	14	178	121	110*
		0.156	52	20	216	139	168*
			37	10	221	197	168*
		0.313	34	3	219	217	241*
			25	3	202	266	241*
		0.625

Positive Control	Ethyl Methane Sulfonate	500	26	13	429	557	552*
Positive Control			42	11	688	546	552*
Negative Control	Negative (Not Specified)	0	132r	150	56	14
Negative Control			103	117	23	7

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	89	46	18	19
			103	106	60	19
			73	88	53	24
			91	117	38	14
Test Chemical	4-Nitroquinoline-N-oxide	0.0078	83	64	145	58	47*
			89	64	98	37	47*
		0.0156	62	67	99	54	52*
			93	78	140	50	52*
		0.0313	83	75	219	88	75*
			79	69	150	63	75*
		0.0625	66	77	190	96
			125r	84	331	88
		0.125	73	56	320	146	142*
			72	48	299	138	142*
		0.25	62	38	429	231	255*
			64	28	537	279	255*
Positive Control	Ethyl Methane Sulfonate	500	40	19	749	624	704*
			37	16	870	777
			43	20	906	711

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	101	181	56	18	20
Vehicle Control			73	55	47	21	20
Test Chemical	4-Nitroquinoline-N-oxide	0.156	64	79	81	42	32
			84	175	57	23	32
		0.313	84	139	78	31	44*
			70	95	122	58	44*
		0.625	60	35	167	93	75*
			88	77	151	57	75*
		1.25	43	47	194	150	155*
			41	21	199	160	155*
		2.5	8r	1	68	272
			25	4	129	170
Positive Control	3-Methylcholanthrene	5	41	21	160	129	127*
Positive Control			54	33	201	125	127*
Negative Control	Negative (Not Specified)	0	94	89	51	18	22*
Negative Control			74	75	56	25	22*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	103	63	28	31
Vehicle Control			62	85	64	35	31
Test Chemical	4-Nitroquinoline-N-oxide	0.156	71	94	110	52	48
			72	104	96	45	48
		0.313	76	133	117	52	53*
			60	90	99	55	53*
		0.625	63	80	146	77	98*
			61	83	220	120	98*
		1.25	56	55	264	158	171*
			52	60	289	183	171*
		2.5	24	3	214	299	347*
			13	2	156	395	347*
Positive Control	3-Methylcholanthrene	5	41	17	323	263	249*
Positive Control			44	19	310	235	249*
Negative Control	Negative (Not Specified)	0	72	103	59	27	27*
Negative Control			74	109	61	28	27*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	91	79	30	30
			68	100	56	27
			88	105	83	32
			65	104	61	31
Test Chemical	4-Nitroquinoline-N-oxide	0.25	56	86	69	41	40
			71	87	83	39	40
		0.5	50	40	82	55	52*
			58	141	86	49	52*
		1	78	107	128	55	71*
			67	91	177	88	71*
		1.5	61	57	134	73	75*
			69	95	158	76	75*
		2	40	52	219	185	154*
			37	45	137	124	154*
		2.5	53	52	247	156	170*
			40	8	218	183	170*
Positive Control	3-Methylcholanthrene	5	50	34	337	225	244*
			54	44	412	255
			48	42	366	252

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination