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Study Information
Study Attribute Value
CEBS Accession Number: 002-01519-0003-0000-1
Chemical Name: 4-Nitroquinoline-N-oxide
CASRN: 56-57-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G23980
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 117 113 64 18 17
    89 70 44 16
Test Chemical 4-Nitroquinoline-N-oxide 
 0.0195    
 113 34 141 41 61*
    52 24 127 81
  
 0.039     
 75 20 221 99 89*
    74 15 176 79
  
 0.078     
 55 28 164 99 110*
    49 14 178 121
  
 0.156     
 52 20 216 139 168*
    37 10 221 197
  
 0.313     
 34 3 219 217 241*
    25 3 202 266
  
 0.625     
   
Positive Control Ethyl Methane Sulfonate 
500         
 26 13 429 557 552*
    42 11 688 546
Negative Control Negative (Not Specified) 
 0         
 132r 150 56 14
    103 117 23 7

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 89 46 18 19
    103 106 60 19
    73 88 53 24
    91 117 38 14
Test Chemical 4-Nitroquinoline-N-oxide 
 0.0078    
 83 64 145 58 47*
    89 64 98 37
  
 0.0156    
 62 67 99 54 52*
    93 78 140 50
  
 0.0313    
 83 75 219 88 75*
    79 69 150 63
  
 0.0625    
 66 77 190 96
    125r 84 331 88
  
 0.125     
 73 56 320 146 142*
    72 48 299 138
  
 0.25      
 62 38 429 231 255*
    64 28 537 279
Positive Control Ethyl Methane Sulfonate 
500         
 40 19 749 624 704*
    37 16 870 777
    43 20 906 711

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 101 181 56 18 20
    73 55 47 21
Test Chemical 4-Nitroquinoline-N-oxide 
 0.156     
 64 79 81 42 32
    84 175 57 23
  
 0.313     
 84 139 78 31 44*
    70 95 122 58
  
 0.625     
 60 35 167 93 75*
    88 77 151 57
  
1.25      
 43 47 194 150 155*
    41 21 199 160
  
2.5       
 8r 1 68 272
    25 4 129 170
Positive Control 3-Methylcholanthrene 
5         
 41 21 160 129 127*
    54 33 201 125
Negative Control Negative (Not Specified) 
 0         
 94 89 51 18 22*
    74 75 56 25

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75 103 63 28 31
    62 85 64 35
Test Chemical 4-Nitroquinoline-N-oxide 
 0.156     
 71 94 110 52 48
    72 104 96 45
  
 0.313     
 76 133 117 52 53*
    60 90 99 55
  
 0.625     
 63 80 146 77 98*
    61 83 220 120
  
1.25      
 56 55 264 158 171*
    52 60 289 183
  
2.5       
 24 3 214 299 347*
    13 2 156 395
Positive Control 3-Methylcholanthrene 
5         
 41 17 323 263 249*
    44 19 310 235
Negative Control Negative (Not Specified) 
 0         
 72 103 59 27 27*
    74 109 61 28

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 91 79 30 30
    68 100 56 27
    88 105 83 32
    65 104 61 31
Test Chemical 4-Nitroquinoline-N-oxide 
 0.25      
 56 86 69 41 40
    71 87 83 39
  
 0.5       
 50 40 82 55 52*
    58 141 86 49
  
1         
 78 107 128 55 71*
    67 91 177 88
  
1.5       
 61 57 134 73 75*
    69 95 158 76
  
2         
 40 52 219 185 154*
    37 45 137 124
  
2.5       
 53 52 247 156 170*
    40 8 218 183
Positive Control 3-Methylcholanthrene 
5         
 50 34 337 225 244*
    54 44 412 255
    48 42 366 252

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination