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Study Information
Study Attribute Value
CEBS Accession Number: 002-01519-0004-0000-2
Chemical Name: 4-Nitroquinoline-N-oxide
CASRN: 56-57-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G39302
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78 105 101 43 45
    68 95 94 46
Test Chemical 4-Nitroquinoline-N-oxide 
 0.0031    
 83 98 162 65 66
    84 102 167 67
  
 0.0063    
 76 99 215 95 102*
    74 96 245 110
  
 0.0125    
 79 98 264 112 110*
    69 95 226 109
  
 0.025     
 68 84 357 176 154*
    68 100 267 131
  
 0.05      
 57 62 346 202 190*
    69 67 368 178
  
 0.1       
 43 38 341 265 280*
    46 38 407 295
Positive Control Ethyl Methane Sulfonate 
500         
 34 30 716 695 681*
    35 33 696 666

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 99 115 151 51 56
    80 85 148 62
Test Chemical 4-Nitroquinoline-N-oxide 
 0.0016    
 84 94 133 53 47
    87 96 107 41
  
 0.0031    
 75 87 132 59 58
    68 76 116 57
  
 0.0063    
 72 88 167 77 73
    73 76 148 68
  
 0.0125    
 74 83 214 96
  
 0.025     
 83 81 267 108 132*
    69 71 320 155
  
 0.1       
 49 30 275 188
Positive Control Ethyl Methane Sulfonate 
500         
 42 27 553 435 446*
    42 27 571 457

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 109 90 34 37
    90 91 110 41
Test Chemical 4-Nitroquinoline-N-oxide 
 0.625     
 96 124 157 54 66*
    82 108 191 78
  
1.25      
 89 109 244 91 87*
    90 102 223 82
  
2.5       
 95 114 285 100 89*
    107 127 250 78
  
5         
 83 87 315 127 144*
    80 76 387 161
  
20         
 23 7 381 564 456*
    25 10 263 348
Positive Control Ethyl Methane Sulfonate 
500         
 702*
    52 40 1088 702

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 93 102 50 18 18
    87 98 46 18
Test Chemical 4-Nitroquinoline-N-oxide 
1.5       
 88 104 68 26 27
    93 113 77 28
  
2.3       
 91 106 65 24 25
    102 121 83 27
  
3.5       
 83 95 108 43 37*
    84 98 78 31
  
5.4       
 83 93 78 32 34*
    84 95 92 37
  
8.3       
 80 88 131 55 48*
    88 93 107 41
  
12.7       
 55 36 120 73 76*
    61 38 145 79
Positive Control 3-Methylcholanthrene 
6         
 72 65 257 119 114*
    72 66 234 109

Non-Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 95 243 99 102
    78 105 246 105
Test Chemical 4-Nitroquinoline-N-oxide 
1.47      
 61 82 227 124
  
2.3       
 71 106 277 130 116
    64 94 195 101
  
3.5       
 59 64 254 143 135
    63 92 242 128
  
5.4       
 56 76 256 153 163
    54 76 281 173
  
8.2       
 48 57 389 272 246*
    45 59 299 221
  
12.7       
 18 10 344 643
    6r 2 238 1322
  
19.5       
   
Positive Control 3-Methylcholanthrene 
6         
 55 55 383 233 222*
    55 54 350 212

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 70 86 102 48 51
    75 114 120 54
Test Chemical 4-Nitroquinoline-N-oxide 
2.3       
 72 88 196 91 93*
    89 112 252 95
  
3.5       
 75 76 328 145
  
5.4       
 45 32 293 219 202*
    49 40 271 185
  
8.2       
 35 16 251 238 318*
    29 12 353 399
  
12.7       
 9r 2 203 725
  
19.5       
   
Positive Control 3-Methylcholanthrene 
5         
 36 31 354 326 341*
    34 35 364 355
3-Methylcholanthrene 
100         
 9r 9 126 475

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 111 105 48 14 18
    106 95 67 21
Test Chemical 4-Nitroquinoline-N-oxide 
 0.96      
 101 103 87 29 25
    93 88 60 22
  
1.5       
 92 97 121 44 42*
    95 90 113 40
  
2.3       
 87 79 152 58 52*
    83 81 115 46
  
3.5       
 76 58 160 70 70*
    76 58 156 69
  
5.4       
 61 35 159 87 87*
    64 38 168 87
  
8.3       
 61 19 237 130 156*
    39 11 211 182
Positive Control 3-Methylcholanthrene 
6         
 59 26 352 199 278*
    38 17 404 356

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination