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Study Information
Study Attribute Value
CEBS Accession Number: 002-01523-0009-0000-2
Chemical Name: 4-Vinylcyclohexene
CASRN: 100-40-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 971117
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 83 86 78 32 33
    88 105 87 33
    72 108 74 34
Test Chemical 4-Vinylcyclohexene 
20         
 56# 58 77 46 47
    46 49 68 49
    46 70 65 47
  
30         
 62 62 64 35 43
    63# 86 103 54
    63# 53 77 41
  
40         
 56# 80 69 41 49
    36# 38 77 72
    72 84 72 33
  
50         
 64 88 35 18 45
    80 93 41 17
    31 35 92 100
  
60         
 62 90 57 31 25
    79 106 44 19
  
80         
 102 114 87 29 35
    70 21 81 38
    62 34 70 38
  
120         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 53# 53 413 258 221*
    57 62 317 187
    50 51 323 218

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 72 95 91 42 38
    69 124 69 33
    74 82 86 39
    64 99 74 39
Test Chemical 4-Vinylcyclohexene 
40         
 46 65 77 56 59*
    43 66 79 61
    60 63 106 59
  
50         
 56 87 48 29 47
    53 75 84 53
    46 51 80 58
  
60         
 45# 54 80 60 47
    47 64 68 48
    39 73 37 32
  
80         
 68 71 82 40 48
    55 52 93 57
    6r 5 115 657
  
100         
 50 42 72 48 48
    44 42 73 55
    70 72 86 41
  
120         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 39 30 331 284 286*
    45 36 451 337
    39 47 275 236

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 96 101 162 56 50
    83# 95 109 44
    98 110 121 41
    79 94 141 59
Test Chemical 4-Vinylcyclohexene 
40         
 91 66 161 59 63
    93 77 187 67
  
50         
 88 68 215 81 74
    81 53 163 67
  
60         
 84 59 185 74 69
    101 62 178 59
    88 56 199 75
  
80         
 83 53 142 57 61
    80 40 155 65
  
100         
 80 35 154 64 79*
    86 33 189 74
    76 29 224 98
  
120         
 29# 4 69 80 92*
    49# 8 134 92
    56 8 173 103
Positive Control 3-Methylcholanthrene 
2.5       
 48 37 523 364 362*
    44 31 582 441
    50# 45 421 282

Induced S9 Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 31r 75 46 50 41
    52 78 53 34
    31r 65 80 86
    63 122 91 48
Test Chemical 4-Vinylcyclohexene 
30         
 24 41 63 89 85*
    25 36 75 101
    41 95 80 65
  
40         
 25 31 78 103 113*
    16 31 63 134
    17 30 52 101
  
50         
 70*
    33 46 62 63
    20 41 47 77
  
60         
 40 44 126 104 102*
    26 35 97 125
    40# 70 90 76
  
80         
 82*
    54 64 102 64
    43 80 131 101
  
100         
 145*
    30 18 126 139
    21 15 96 151
  
120         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 26 13 502 648 557*
    25 15 411 541
    23 8 338 483

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 77 70 218 94 70
    73 95 108 49
    74 107 151 68
    70 128 145 70
Test Chemical 4-Vinylcyclohexene 
40         
 58 44 127 73 61
    57 62 127 75
    52 69 54 35
  
50         
 57 54 129 75 83
    46 56 111 81
    58 64 161 93
  
60         
 47 47 146 105 112*
    53 57 173 108
    40 37 147 123
  
80         
 61 29 261 143 149*
    37 38 227 203
    55 53 168 102
  
100         
 58 58 161 93 108*
    69 67 199 96
    45 10 184 136
  
120         
 50 39 229 154 148*
    76 40 303 133
    38 35 177 157
  
150         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 54 65 430 265 245*
    62 64 472 253
    76 87 494 218

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination