Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01523-0009-0000-2
Chemical Name:	4-Vinylcyclohexene
CASRN:	100-40-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	971117
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	83	86	78	32	33
			88	105	87	33
			72	108	74	34
Test Chemical	4-Vinylcyclohexene	20	56#	58	77	46	47
			46	49	68	49
			46	70	65	47
		30	62	62	64	35	43
			63#	86	103	54
			63#	53	77	41
		40	56#	80	69	41	49
			36#	38	77	72
			72	84	72	33
		50	64	88	35	18	45
			80	93	41	17
			31	35	92	100
		60	62	90	57	31	25
			79	106	44	19	25
		80	102	114	87	29	35
			70	21	81	38
			62	34	70	38
		120


Positive Control	Methyl Methane Sulfonate	5	53#	53	413	258	221*
			57	62	317	187
			50	51	323	218

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	72	95	91	42	38
			69	124	69	33
			74	82	86	39
			64	99	74	39
Test Chemical	4-Vinylcyclohexene	40	46	65	77	56	59*
			43	66	79	61
			60	63	106	59
		50	56	87	48	29	47
			53	75	84	53
			46	51	80	58
		60	45#	54	80	60	47
			47	64	68	48
			39	73	37	32
		80	68	71	82	40	48
			55	52	93	57
			6r	5	115	657
		100	50	42	72	48	48
			44	42	73	55
			70	72	86	41
		120


Positive Control	Methyl Methane Sulfonate	5	39	30	331	284	286*
			45	36	451	337
			39	47	275	236

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	96	101	162	56	50
			83#	95	109	44
			98	110	121	41
			79	94	141	59
Test Chemical	4-Vinylcyclohexene	40	91	66	161	59	63
			93	77	187	67	63
		50	88	68	215	81	74
			81	53	163	67	74
		60	84	59	185	74	69
			101	62	178	59
			88	56	199	75
		80	83	53	142	57	61
			80	40	155	65	61
		100	80	35	154	64	79*
			86	33	189	74
			76	29	224	98
		120	29#	4	69	80	92*
			49#	8	134	92
			56	8	173	103
Positive Control	3-Methylcholanthrene	2.5	48	37	523	364	362*
			44	31	582	441
			50#	45	421	282

Induced S9 Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	31r	75	46	50	41
			52	78	53	34
			31r	65	80	86
			63	122	91	48
Test Chemical	4-Vinylcyclohexene	30	24	41	63	89	85*
			25	36	75	101
			41	95	80	65
		40	25	31	78	103	113*
			16	31	63	134
			17	30	52	101
		50					70*
			33	46	62	63
			20	41	47	77
		60	40	44	126	104	102*
			26	35	97	125
			40#	70	90	76
		80					82*
			54	64	102	64
			43	80	131	101
		100					145*
			30	18	126	139
			21	15	96	151
		120


Positive Control	Methyl Methane Sulfonate	5	26	13	502	648	557*
			25	15	411	541
			23	8	338	483

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	77	70	218	94	70
			73	95	108	49
			74	107	151	68
			70	128	145	70
Test Chemical	4-Vinylcyclohexene	40	58	44	127	73	61
			57	62	127	75
			52	69	54	35
		50	57	54	129	75	83
			46	56	111	81
			58	64	161	93
		60	47	47	146	105	112*
			53	57	173	108
			40	37	147	123
		80	61	29	261	143	149*
			37	38	227	203
			55	53	168	102
		100	58	58	161	93	108*
			69	67	199	96
			45	10	184	136
		120	50	39	229	154	148*
			76	40	303	133
			38	35	177	157
		150


Positive Control	Methyl Methane Sulfonate	5	54	65	430	265	245*
			62	64	472	253
			76	87	494	218

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination