Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01525-0012-0000-8 |
Chemical Name: | 5,5-Diphenylhydantoin (phenytoin) |
CASRN: | 57-41-0 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 404495 |
Study Result: | Negative |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 70 | 104 | 65 | 31 | 26 |
83 | 112 | 44 | 18 | ||||
73 | 94 | 63 | 29 | ||||
84 | 90 | 69 | 27 | ||||
Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 31.25 | 59 | 84 | 46 | 26 | 28 |
70 | 96 | 61 | 29 | ||||
62.5 | 75 | 84 | 84 | 37 | 34 | ||
69 | 84 | 62 | 30 | ||||
125 | 57 | 52 | 51 | 30 | 33 | ||
54 | 46 | 58 | 36 | ||||
250 | 50 | 32 | 74 | 50 | 36 | ||
47 | 32 | 30 | 21 | ||||
500 | 56 | 31 | 58 | 35 | 30 | ||
46 | 13 | 35 | 25 | ||||
Positive Control | Methyl Methane Sulfonate | 15 | 46 | 39 | 106 | 77 | 88* |
35 | 35 | 103 | 99 | ||||
Methyl Methane Sulfonate | 250 | 34 | 47 | 271 | 266 | 270* | |
37 | 42 | 304 | 274 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 74 | 89 | 84 | 38 | 38 |
85 | 106 | 121 | 48 | ||||
85 | 98 | 90 | 35 | ||||
99 | 106 | 92 | 31 | ||||
Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 15 | 76 | 95 | 101 | 44 | 43 |
76 | 100 | 96 | 42 | ||||
30 | 78 | 88 | 99 | 42 | 38 | ||
78 | 79 | 80 | 34 | ||||
60 | 70 | 74 | 112 | 54 | 53 | ||
65 | 60 | 103 | 53 | ||||
120 | 52 | 35 | 91 | 58 | 53 | ||
58 | 21 | 83 | 48 | ||||
240 | 58 | 22 | 169 | 96 | 90* | ||
65 | 29 | 162 | 84 | ||||
Positive Control | Methyl Methane Sulfonate | 15 | 29 | 17 | 169 | 195 | 205* |
31 | 19 | 199 | 214 | ||||
Methyl Methane Sulfonate | 250 | 57 | 52 | 540 | 316 | 312* | |
68 | 62 | 631 | 308 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 52 | 85 | 65 | 41 | 40 |
50 | 74 | 80 | 53 | ||||
81 | 124 | 75 | 31 | ||||
78 | 118 | 77 | 33 | ||||
Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 17.5 | 41 | 58 | 62 | 51 | 53 |
50 | 77 | 82 | 55 | ||||
35 | 46 | 73 | 46 | 33 | 40 | ||
49 | 72 | 70 | 47 | ||||
70 | 64 | 68 | 62 | 33 | 37 | ||
72 | 54 | 91 | 42 | ||||
140 | 67 | 36 | 56 | 28 | 34 | ||
66 | 31 | 79 | 40 | ||||
280 | 73 | 32 | 76 | 35 | 39 | ||
58 | 28 | 74 | 43 | ||||
Positive Control | Methyl Methane Sulfonate | 20 | 7r | 4 | 139 | 647 | |
6r | 4 | 113 | 611 | ||||
Methyl Methane Sulfonate | 250 | 56 | 58 | 648 | 387 | 387* | |
48 | 53 | 555 | 388 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 86 | 90 | 58 | 22 | 24 |
116 | 88 | 105 | 30 | ||||
95 | 114 | 64 | 22 | ||||
106 | 107 | 61 | 19 | ||||
Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 17.5 | 102 | 105 | 75 | 25 | 28 |
93 | 77 | 89 | 32 | ||||
35 | 107 | 85 | 98 | 31 | 27 | ||
101 | 79 | 73 | 24 | ||||
70 | 101 | 78 | 70 | 23 | 30 | ||
114 | 85 | 125 | 37 | ||||
140 | 90 | 27 | 86 | 32 | 32 | ||
77 | 22 | 74 | 32 | ||||
280 | 88 | 12 | 69 | 26 | 26 | ||
85 | 11 | 67 | 26 | ||||
Positive Control | Methyl Methane Sulfonate | 15 | 29 | 17 | 219 | 249 | 241* |
37 | 18 | 256 | 234 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 84 | 103 | 88 | 35 | 32 |
83 | 104 | 68 | 27 | ||||
86 | 94 | 84 | 32 | ||||
70 | 99 | 72 | 35 | ||||
Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 18.75 | 83 | 85 | 80 | 32 | 32 |
84 | 94 | 80 | 32 | ||||
37.5 | 72 | 112 | 64 | 30 | 29 | ||
76 | 106 | 66 | 29 | ||||
75 | 86 | 80 | 88 | 34 | 35 | ||
72 | 58 | 79 | 37 | ||||
150 | 73 | 48 | 76 | 35 | 29 | ||
89 | 43 | 60 | 23 | ||||
300 | 78 | 15 | 74 | 32 | 28 | ||
63 | 14 | 47 | 25 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 50 | 32 | 477 | 319 | 360* |
41 | 28 | 490 | 400 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 76 | 91 | 50 | 22 | 25 |
90 | 103 | 118 | 44 | ||||
81 | 98 | 47 | 19 | ||||
75 | 108 | 31 | 14 | ||||
Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 50 | 102 | 123 | 64 | 21 | 24 |
82 | 107 | 66 | 27 | ||||
100 | 74 | 49 | 56 | 25 | 26 | ||
85 | 31 | 70 | 28 | ||||
150 | 99 | 34 | 55 | 19 | 19 | ||
64 | 21 | 36 | 19 | ||||
200 | 81 | 66 | 47 | 19 | 24 | ||
96 | 51 | 85 | 30 | ||||
250 | 93 | 41 | 58 | 21 | 28 | ||
90# | 66 | 96 | 35 | ||||
300 | 59 | 22 | 68 | 38 | 33 | ||
69 | 32 | 56 | 27 | ||||
350 | 79# | 21 | 108 | 46 | 36 | ||
88 | 26 | 70 | 26 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 66 | 43 | 479 | 241 | 223* |
56 | 36 | 344 | 205 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination