Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01528-0001-0000-9
Chemical Name:	5,6-Dihydro-5-azacytidine
CASRN:	62488-57-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	450014
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	100	89	57	19	19
			91	118	41	15
			94	95	58	21
			84	98	55	22
Test Chemical	5,6-Dihydro-5-azacytidine	1.5	87	77	61	23	21
			92	92	51	19	21
		4.5	91	83	85	31	32*
			75	68	72	32	32*
		15	69	43	114	55	48*
			80	53	98	41	48*
		45	48	21	45	32	40*
			40	22	59	49	40*
		150	45	8	43	32	35*
			34	5	38	38	35*
		450

Positive Control	Methyl Methane Sulfonate	15	42	25	178	142	145*
Positive Control			42	19	188	148	145*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	98	96	43	15	16
			93	109	25	9
			102	96	92	30
			92#	100	32	12
Test Chemical	5,6-Dihydro-5-azacytidine	1.5	84	80	73	29	32*
			101	96	107	35	32*
		4.5	68	74	83	41	42*
			85	77	111	43	42*
		15	52	45	99	63	67*
			42	45	88	70	67*
		45	22	9	49	74	76*
			29	10	68	78	76*
		150	7r	1	23	107
			6r	0	11	59
		450

Positive Control	Methyl Methane Sulfonate	15	39	25	118	101	129*
Positive Control			32	20	150	157	129*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	70	97	44	21	23
			75	108	56	25
			69	98	44	21
			68	97	47	23
Test Chemical	5,6-Dihydro-5-azacytidine	0.25	66#	81	56	28	26
			75	81	51	23	26
		0.5	75	95	63	28	26
			70	95	50	24	26
		1	70	78	80	38	35
			65	94	62	32	35
		2	69	71	94	46	43*
			60	78	73	40	43*
		4	60	71	115	64	62*
			71	75	127	60	62*
		8	60	68	130	73	72*
			62	65	133	72	72*
Positive Control	Methyl Methane Sulfonate	15	26	19	158	207	215*
Positive Control			28	21	184	223	215*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination