Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01535-0001-0000-7
Chemical Name:	5-Aza-2'-deoxycytidine
CASRN:	2353-33-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	635626
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	105	84	35	32
			78	92	76	32
			60	95	57	32
			65	108	59	30
Test Chemical	5-Aza-2'-deoxycytidine	3.13	27	22	251	306	380*
			24	21	327	454	380*
		6.25	18	13	318	578	572*
			20	15	337	566	572*
		12.5	22	11	326	487	539*
			19	10	340	591	539*
		25	21	15	336	529	524*
			22	14	337	518	524*
		50	21	13	360	581	546*
			17	8	263	511	546*
		100	26	15	336	434	510*
			13	6	226	587	510*
Positive Control	Methyl Methane Sulfonate	15	33	21	349	349	383*
Positive Control			32	20	394	417	383*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	117	142	51	41
			86	116	110	43
			60	81	55	31
			58	86	68	39
Test Chemical	5-Aza-2'-deoxycytidine	3.13	19	10	373	672	874*
			17	9	533	1077	874*
		6.25	16	7	424	883	924*
			16	6	463	965	924*
		12.5	7r	3	310	1550
			13	5	408	1060
		25	9r	3	329	1265
			7r	3	293	1465
		50	7r	2	257	1195
			6r	2	251	1521
		100	6r	1	189	1022
			6r	1	201	1149
Positive Control	Methyl Methane Sulfonate	15	18	12	224	407	339*
Positive Control			27	19	221	271	339*

Nonactivation Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	83	68	27	22
			104	108	71	23
			97	108	59	20
			96	102	56	19
Test Chemical	5-Aza-2'-deoxycytidine	0.001	102	101	112	37	28
			77	93	45	19	28
		0.003	85	95	62	24	27
			71	85	63	30	27
		0.01	72	88	117	54	61*
			71	74	145	68	61*
		0.03	83	54	235	94	98*
			89	61	271	101	98*
		0.1	64	32	259	136	148*
			56	29	269	160	148*
		0.3	38	19	302	267	254*
			33	12	241	241	254*
Positive Control	Methyl Methane Sulfonate	15	49	22	169	115	144*
Positive Control			46	31	237	174	144*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination