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Study Information
Study Attribute Value
CEBS Accession Number: 002-01536-0002-0000-9
Chemical Name: 5-Azacytidine
CASRN: 320-67-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 932806_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Positive 
No Activation  3  Positive 

Trial #:1   Activation: No Activation    Date: 04/02/1985    Harvest Time: 12.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 5-Azacytidine 
3         
 100 3 0.030 2.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
5         
 100 14 0.140 13.0 5 0.050 5.0 9 0.090 8.0 0 0.000 0.0 
10         
 100 22 0.220 20.0 6 0.060 4.0 16 0.160 16.0 0 0.000 0.0 
16         
 100 31 0.310 26.0 9 0.090 7.0 22 0.220 20.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 19 0.190 18.0 11 0.110 11.0 8 0.080 7.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 13 0.260 24.0 8 0.160 14.0 5 0.100 10.0 0 0.000 0.0
Trend: 6.289 1.890 6.104  
Probability: 0.000 0.029 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/02/1985    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 4 0.040 3.0 0 0.000 0.0 4 0.040 3.0 0 0.000 0.0
Test Chemical: 5-Azacytidine 
3         
 100 6 0.060 3.0 3 0.030 2.0 3 0.030 3.0 0 0.000 0.0 
5         
 100 5 0.050 5.0 0 0.000 0.0 5 0.050 5.0 0 0.000 0.0 
10         
 100 2 0.020 1.0 2 0.020 1.0 0 0.000 0.0 0 0.000 0.0 
16         
 100 4 0.040 2.0 1 0.010 1.0 3 0.030 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 100 65 0.650 40.0 32 0.320 27.0 23 0.230 18.0 10 0.100 1.0
Cyclophosphamide 
15         
 50 35 0.700 50.0 30 0.600 44.0 5 0.100 8.0 0 0.000 0.0
Trend: -0.842 0.384 -1.543  
Probability: 0.800 0.351 0.939  


Trial #:2   Activation: No Activation    Date: 04/02/1985    Harvest Time: 22.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 5-Azacytidine 
30         
 100 10 0.100 9.0 6 0.060 5.0 4 0.040 4.0 0 0.000 0.0 
50         
 100 22 0.220 17.0 13 0.130 11.0 9 0.090 8.0 0 0.000 0.0 
100         
 100 118 1.180 78.0 97 0.970 61.0 21 0.210 21.0 0 0.000 0.0 
160         
 100 148 1.480 83.0 113 1.130 65.0 35 0.350 30.0 0 0.000 0.0 
300         
 4 10 2.500 100.0 8 2.000 100.0 2 0.500 25.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 29 0.290 24.0 12 0.120 12.0 17 0.170 15.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 35 0.700 52.0 23 0.460 38.0 12 0.240 24.0 0 0.000 0.0
Trend: 15.476 13.208 7.350  
Probability: 0.000 0.000 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 04/02/1985    Harvest Time: 18 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 5-Azacytidine 
100         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
160         
 100 12 0.120 11.0 5 0.050 4.0 7 0.070 7.0 0 0.000 0.0 
300         
 100 84 0.840 52.0 45 0.450 29.0 39 0.390 32.0 0 0.000 0.0 
500         
 100 136 1.360 74.0 92 0.920 59.0 44 0.440 35.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 100 126 1.260 68.0 72 0.720 47.0 44 0.440 37.0 10 0.100 1.0
Cyclophosphamide 
15         
 50 97 1.940 88.0 63 1.260 72.0 34 0.680 48.0 0 0.000 0.0
Trend: 13.452 11.570 8.611  
Probability: 0.000 0.000 0.000  


Trial #:3   Activation: No Activation    Date: 06/05/1985    Harvest Time: 14 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 5-Azacytidine 
3         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5         
 100 5 0.050 5.0 0 0.000 0.0 5 0.050 5.0 0 0.000 0.0 
10         
 100 8 0.080 8.0 0 0.000 0.0 8 0.080 8.0 0 0.000 0.0 
16         
 100 50 0.500 39.0 10 0.100 7.0 40 0.400 39.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 20 0.200 19.0 15 0.150 15.0 5 0.050 5.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 31 0.620 44.0 16 0.320 26.0 15 0.300 24.0 0 0.000 0.0
Trend: 8.490 3.677 8.490  
Probability: 0.000 0.000 0.000