Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01536-0010-0000-8
Chemical Name:	5-Azacytidine
CASRN:	320-67-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	010439
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	88	109	50	47
			81	112	114	47
			61	100	75	41
			69	101	105	51
Test Chemical	5-Azacytidine	0.5	5r	3	95	594
			6r	4	88	518
		1	2r	1	127	2309
			3r	1	139	1853
		2	1r	0	306	7650
			3r	1	361	4247
		4	2r	0	609	13533
			3r	0	683	9107
		8	4r	1	779	6774
			4r	0	883	7678
		16

Positive Control	Methyl Methane Sulfonate	15	26	21	168	218	201*
			33	21	183	184	201*
	Methyl Methane Sulfonate	250	55	59	327	197	189*
			64	76	348	180	189*

Nonactivation Trial: 2 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	98	56	20	20
			93	108	54	19
			81	93	60	25
			90	101	48	18
Test Chemical	5-Azacytidine	2	5r	1	32	237
			3r	1	34	378
		4	4r	1	57	496
			2r	0	44	733
		6	3r	0	55	687
			5r	1	99	660
		8	5r	0	125	833
			2r	0	86	1564
		10	4r	0	116	1055
			5r	0	109	807
		12

Positive Control	Methyl Methane Sulfonate	15	42	27	141	111	117*
			47	26	174	124	117*
	Methyl Methane Sulfonate	250	79	56	362	153	188*
			82	68	548	224	188*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	91	88	33	39
			98#	95	127.5	43
			98	115	120	41
			92#	100	102	37
Test Chemical	5-Azacytidine	0.01	96	103	161	56	56
			102	117	174	57	56
		0.033	90	76	252	93	96*
			98	105	292	100	96*
		0.1	92#	122	322.5	117	115*
			81	74	274	113	115*
		0.33	46	25	244	177	180*
			48	27	264	183	180*
		1	12	6	258	717
			10r	5	287	973
		3.33	2r	0	111	2220
			2r	0	157	3140
Positive Control	Methyl Methane Sulfonate	15	42	33	512	408	351*
			45	29	395	294	351*
	Methyl Methane Sulfonate	250	95	87	579	203	212*
			91	104	600	221	212*

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70	97	27	13	16
			82	105	65	26
			89	106	32	12
			70	92	25	12
Test Chemical	5-Azacytidine	0.01	85	101	60	24	32*
			99	111	120	40	32*
		0.033	70	81	150	72	65*
			62	73	107	58	65*
		0.1	55	49	134	82	80*
			60	63	140	78	80*
		0.33	31	19	94	102	114*
			30	19	112	126	114*
		1	16	7	58	125	129*
			12	6	49	132	129*
		3.3	1r	0	41	2733
			1r	0	28	1867
Positive Control	Methyl Methane Sulfonate	20	17	9	53	105	107*
Positive Control			20	10	66	110	107*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination