Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01536-0010-0000-8 |
Chemical Name: | 5-Azacytidine |
CASRN: | 320-67-2 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 010439 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 73 | 88 | 109 | 50 | 47 |
81 | 112 | 114 | 47 | ||||
61 | 100 | 75 | 41 | ||||
69 | 101 | 105 | 51 | ||||
Test Chemical | 5-Azacytidine | 0.5 | 5r | 3 | 95 | 594 | |
6r | 4 | 88 | 518 | ||||
1 | 2r | 1 | 127 | 2309 | |||
3r | 1 | 139 | 1853 | ||||
2 | 1r | 0 | 306 | 7650 | |||
3r | 1 | 361 | 4247 | ||||
4 | 2r | 0 | 609 | 13533 | |||
3r | 0 | 683 | 9107 | ||||
8 | 4r | 1 | 779 | 6774 | |||
4r | 0 | 883 | 7678 | ||||
16 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 26 | 21 | 168 | 218 | 201* |
33 | 21 | 183 | 184 | ||||
Methyl Methane Sulfonate | 250 | 55 | 59 | 327 | 197 | 189* | |
64 | 76 | 348 | 180 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 93 | 98 | 56 | 20 | 20 |
93 | 108 | 54 | 19 | ||||
81 | 93 | 60 | 25 | ||||
90 | 101 | 48 | 18 | ||||
Test Chemical | 5-Azacytidine | 2 | 5r | 1 | 32 | 237 | |
3r | 1 | 34 | 378 | ||||
4 | 4r | 1 | 57 | 496 | |||
2r | 0 | 44 | 733 | ||||
6 | 3r | 0 | 55 | 687 | |||
5r | 1 | 99 | 660 | ||||
8 | 5r | 0 | 125 | 833 | |||
2r | 0 | 86 | 1564 | ||||
10 | 4r | 0 | 116 | 1055 | |||
5r | 0 | 109 | 807 | ||||
12 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 42 | 27 | 141 | 111 | 117* |
47 | 26 | 174 | 124 | ||||
Methyl Methane Sulfonate | 250 | 79 | 56 | 362 | 153 | 188* | |
82 | 68 | 548 | 224 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 88 | 91 | 88 | 33 | 39 |
98# | 95 | 127.5 | 43 | ||||
98 | 115 | 120 | 41 | ||||
92# | 100 | 102 | 37 | ||||
Test Chemical | 5-Azacytidine | 0.01 | 96 | 103 | 161 | 56 | 56 |
102 | 117 | 174 | 57 | ||||
0.033 | 90 | 76 | 252 | 93 | 96* | ||
98 | 105 | 292 | 100 | ||||
0.1 | 92# | 122 | 322.5 | 117 | 115* | ||
81 | 74 | 274 | 113 | ||||
0.33 | 46 | 25 | 244 | 177 | 180* | ||
48 | 27 | 264 | 183 | ||||
1 | 12 | 6 | 258 | 717 | |||
10r | 5 | 287 | 973 | ||||
3.33 | 2r | 0 | 111 | 2220 | |||
2r | 0 | 157 | 3140 | ||||
Positive Control | Methyl Methane Sulfonate | 15 | 42 | 33 | 512 | 408 | 351* |
45 | 29 | 395 | 294 | ||||
Methyl Methane Sulfonate | 250 | 95 | 87 | 579 | 203 | 212* | |
91 | 104 | 600 | 221 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 70 | 97 | 27 | 13 | 16 |
82 | 105 | 65 | 26 | ||||
89 | 106 | 32 | 12 | ||||
70 | 92 | 25 | 12 | ||||
Test Chemical | 5-Azacytidine | 0.01 | 85 | 101 | 60 | 24 | 32* |
99 | 111 | 120 | 40 | ||||
0.033 | 70 | 81 | 150 | 72 | 65* | ||
62 | 73 | 107 | 58 | ||||
0.1 | 55 | 49 | 134 | 82 | 80* | ||
60 | 63 | 140 | 78 | ||||
0.33 | 31 | 19 | 94 | 102 | 114* | ||
30 | 19 | 112 | 126 | ||||
1 | 16 | 7 | 58 | 125 | 129* | ||
12 | 6 | 49 | 132 | ||||
3.3 | 1r | 0 | 41 | 2733 | |||
1r | 0 | 28 | 1867 | ||||
Positive Control | Methyl Methane Sulfonate | 20 | 17 | 9 | 53 | 105 | 107* |
20 | 10 | 66 | 110 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination