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Study Information
Study Attribute Value
CEBS Accession Number: 002-01540-0001-0000-3
Chemical Name: 5-Fluoro-2'-deoxycytidine
CASRN: 10356-76-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 519438
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 71 101 120 56 54
    57 88 124 73
    66 100 86 44
    77 111 99 43
Test Chemical 5-Fluoro-2'-deoxycytidine 
 0.25      
 67 43 699 348 348*
    55 43 570 348
  
 0.5       
 57 25 686 400 389*
    53 35 599 378
  
1         
 51 15 596 393 419*
    45 14 603 445
  
2         
 29 5 450 511 513*
    34 6 528 515
  
4         
 13 1 1458 3837 2185*
    19 2 307 534
  
8         
   
Positive Control Methyl Methane Sulfonate 
15         
 22 13 224 345 288*
    44 40 304 231

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 83 101 61 24 36
    74 91 88 40
    65 104 90 46
    76 104 74 33
Test Chemical 5-Fluoro-2'-deoxycytidine 
 0.031     
 48 33 338 233 237*
    51 29 370 242
  
 0.063     
 63 32 477 251 232*
    49 33 316 214
  
 0.125     
 50 26 581 387 365*
    62 29 641 343
  
 0.25      
 43 19 615 475 466*
    43 19 584 456
  
 0.5       
 36 6 589 548 511*
    39 8 558 475
  
1         
 21 2 421 685 614*
    32 7 521 543
Positive Control Methyl Methane Sulfonate 
15         
 23 20 213 304 289*
    17 14 140 275

Nonactivation Trial: 3 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 90 86 30 11 20
    74 106 46 21
    92 101 47 17
    87 107 79 30
Test Chemical 5-Fluoro-2'-deoxycytidine 
 0.001     
 81 80 55 23 28
    90 76 90 34
  
 0.003     
 83 72 162 65 62*
    94 61 165 58
  
 0.01      
 63 48 177 94 92*
    59 52 158 89
  
 0.03      
 57 38 237 138 137*
    64 33 262 136
  
 0.1       
 50 39 235 158 160*
    49 22 236 161
  
 0.3       
 60 22 358 198 204*
    72 22 451 209
Positive Control Methyl Methane Sulfonate 
15         
 18 15 142 265 226*
    31 23 175 187

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination