Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01540-0001-0000-3
Chemical Name:	5-Fluoro-2'-deoxycytidine
CASRN:	10356-76-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	519438
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	71	101	120	56	54
			57	88	124	73
			66	100	86	44
			77	111	99	43
Test Chemical	5-Fluoro-2'-deoxycytidine	0.25	67	43	699	348	348*
			55	43	570	348	348*
		0.5	57	25	686	400	389*
			53	35	599	378	389*
		1	51	15	596	393	419*
			45	14	603	445	419*
		2	29	5	450	511	513*
			34	6	528	515	513*
		4	13	1	1458	3837	2185*
			19	2	307	534	2185*
		8

Positive Control	Methyl Methane Sulfonate	15	22	13	224	345	288*
Positive Control			44	40	304	231	288*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	83	101	61	24	36
			74	91	88	40
			65	104	90	46
			76	104	74	33
Test Chemical	5-Fluoro-2'-deoxycytidine	0.031	48	33	338	233	237*
			51	29	370	242	237*
		0.063	63	32	477	251	232*
			49	33	316	214	232*
		0.125	50	26	581	387	365*
			62	29	641	343	365*
		0.25	43	19	615	475	466*
			43	19	584	456	466*
		0.5	36	6	589	548	511*
			39	8	558	475	511*
		1	21	2	421	685	614*
			32	7	521	543	614*
Positive Control	Methyl Methane Sulfonate	15	23	20	213	304	289*
Positive Control			17	14	140	275	289*

Nonactivation Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	90	86	30	11	20
			74	106	46	21
			92	101	47	17
			87	107	79	30
Test Chemical	5-Fluoro-2'-deoxycytidine	0.001	81	80	55	23	28
			90	76	90	34	28
		0.003	83	72	162	65	62*
			94	61	165	58	62*
		0.01	63	48	177	94	92*
			59	52	158	89	92*
		0.03	57	38	237	138	137*
			64	33	262	136	137*
		0.1	50	39	235	158	160*
			49	22	236	161	160*
		0.3	60	22	358	198	204*
			72	22	451	209	204*
Positive Control	Methyl Methane Sulfonate	15	18	15	142	265	226*
Positive Control			31	23	175	187	226*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination