COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01546-0001-0000-9
Chemical Name: 5-Nitro-o-anisidine
CASRN: 99-59-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 509998_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 02/17/1987    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 420 0.400 8.40 26.0 0.000
Test Chemical 5-Nitro-o-anisidine 
1.6       
 50 1049 390 0.372 7.80 26.0 -7.143 
5         
 50 1049 422 0.402 8.44 26.0 0.476 
16         
 50 1050 429 0.409 8.58 26.0 2.046 
50         
 50 1046 469 0.448 9.38 26.0 11.987 
160         
 5 105 47 0.448 9.40 26.0 11.798
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 602 0.574 12.04 26.0 43.333
Mitomycin-C 
 0.005     
 10 210 301 1.433 30.10 26.0 257.992
Trend: 2.226        
Probability: 0.013        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/17/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 378 0.360 7.56 26.0 0.000
Test Chemical 5-Nitro-o-anisidine 
160         
 50 1052 439 0.417 8.78 26.0 15.917 
500         
 50 1050 455 0.433 9.10 26.0 20.370 
1600         
 50 1050 540 0.514 10.80 26.0 42.857 
4000         
 50 1051 524 0.499 10.48 26.0 38.492
Positive Control Cyclophosphamide 
 0.1       
 50 1051 531 0.505 10.62 26.0 40.343
Cyclophosphamide 
 0.6       
 10 212 216 1.019 21.60 26.0 183.019
Trend: 5.806        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 04/21/1987    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 493 0.470 9.86 26.0 0.000
Test Chemical 5-Nitro-o-anisidine 
160         
 50 1049 544 0.519 10.88 26.0 10.345 
500         
 50 1050 514 0.490 10.28 26.0 4.160 
1600         
 50 1047 566 0.541 11.32 26.0 15.027 
4000         
 50 1050 634 0.604 12.68 26.0 28.478
Positive Control Cyclophosphamide 
 0.1       
 50 1048 600 0.573 12.00 26.0 21.820
Cyclophosphamide 
 0.6       
 10 210 200 0.952 20.00 26.0 102.647
Trend: 4.039        
Probability: 0.000