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Study Information
Study Attribute Value
CEBS Accession Number: 002-01547-0002-0000-1
Chemical Name: 5-Nitro-o-toluidine
CASRN: 99-55-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 012878_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #:1   Activation: No Activation    Date: 06/10/1987    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 5-Nitro-o-toluidine 
160         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
300         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
500         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 33 0.165 15.0 15 0.075 7.0 18 0.090 8.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 25 0.500 28.0 8 0.160 14.0 7 0.140 14.0 10 0.200 1.0
Trend: 1.420 0.000 1.420  
Probability: 0.078 0.000 0.078  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 06/10/1987    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: 5-Nitro-o-toluidine 
300         
 200 6 0.030 2.0 1 0.005 0.5 5 0.025 1.5 0 0.000 0.0 
500         
 200 17 0.085 7.0 5 0.025 1.5 12 0.060 5.5 0 0.000 0.0 
1000         
 40 44 1.100 97.5 3 0.075 7.5 41 1.025 95.0 0 0.000 0.0 
3000         
 200 260 1.300 88.0 5 0.025 2.0 177 0.885 80.0 78 0.390 15.0 
5000         
 200 96 0.480 33.0 17 0.085 7.5 65 0.325 28.5 14 0.070 5.0
Positive Control: Cyclophosphamide 
2.5       
 200 45 0.225 19.0 15 0.075 7.5 30 0.150 13.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 20 0.400 30.0 4 0.080 8.0 16 0.320 24.0 0 0.000 0.0
Trend: 17.784 4.606 16.502  
Probability: 0.000 0.000 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 07/23/1987    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 5-Nitro-o-toluidine 
750         
 200 135 0.675 63.5 6 0.030 3.0 121 0.605 57.0 8 0.040 8.0 
1600         
 200 145 0.725 57.0 10 0.050 5.0 100 0.500 48.0 35 0.175 8.0 
3000         
 200 289 1.445 59.0 6 0.030 2.5 80 0.400 37.0 203 1.015 41.0 
5000         
 200 74 0.370 22.0 12 0.060 4.5 38 0.190 18.0 24 0.120 6.0
Positive Control: Cyclophosphamide 
2.5       
 200 33 0.165 14.5 4 0.020 2.0 29 0.145 13.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 18 0.360 32.0 3 0.060 6.0 15 0.300 28.0 0 0.000 0.0
Trend: 4.143 2.259 1.890  
Probability: 0.000 0.012 0.029