Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01554-0011-0000-9
Chemical Name:	6-Methoxy-2-benzothiazolamine
CASRN:	1747-60-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	861345
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	105	111	86	27	30
			104	106	85	27
			95	103	84	29
			81	80	86	36
Test Chemical	6-Methoxy-2-benzothiazolamine	100	105	82	101	32	33
			103	92	108	35
			111	75	102	31
		200	79	50	106	45	38
			89	64	92	34
			104	66	106	34
		300	93	54	87	31	29
			90	47	78	29
			100	50	77	26
		400	90	34	190	71	59*
			121r	32	268	74
			120	46	168	47
		600


Positive Control	Methyl Methane Sulfonate	5	58	55	425	245	266*
			54	43	450	277
			57	48	472	275

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	119#	99	91	26	27
			97#	102	71	24
			110	107	114	35
			106#	92	77	24
Test Chemical	6-Methoxy-2-benzothiazolamine	100	102	79	103	34	32
			117	89	97	28
			120	75	121	34
		200	103	82	101	33	32
			117	75	119	34
			109	68	97	30
		300	112	40	100	30	36
			110	33	131	40
			86	16	102	40
		400	112#	23	155	46	44*
			96#	33	122	42
			121r	36	81	22
		600	110	46	60	18
			122r	34	136	37
		800


Positive Control	Methyl Methane Sulfonate	5	81	56	973	399	384*
			87	59	919	353
			71	46	854	401

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	104	103	125	40	40
			124r	82	154	42
			110	94	148	45
			95	102	103	36
Test Chemical	6-Methoxy-2-benzothiazolamine	50	95	62	101	35	42
			101	81	106	35
			83	56	141	57
		75	110	46	121	37	38
			114	51	173	51
			106	40	89	28
		100	87	44	120	46	53
			122r	60	161	44
			106	44	189	59
		150	118	35	188	53	53
			88	17	141	53
			93r	10	211	76
		200

		300


Positive Control	Methyl Methane Sulfonate	5	86	42	1146	447	408*
			85	50	1092	429
			66	42	684	347

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	98	109	35	34
			96	113	123	43
			101	95	78	26
			92	94	86	31
Test Chemical	6-Methoxy-2-benzothiazolamine	0.625	120	70	143	40	44
			117	92	177	50
			95	80	119	42
		1.25	110	81	185	56	53*
			117	75	162	46
			106	89	178	56
		2.5	126r	87	215	57	87*
			112	59	302	90
			116#	58	293	85
		5	95	21	300	105	189*
			63	9	531	281
			73	12	400	183
		7.5	70	8	409	194	225*
			76	8	456	200
			68	6	573	281
		10	65	5	574	297	341*
			48	3	437	301
			38	3	480	425
		15


Positive Control	3-Methylcholanthrene	2.5	101	74	608	201	218*
			96	67	509	177
			92	65	755	275

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	101	105	105	35	28
			105#	88	88	28
			101	95	81	27
			92	113	67	24
Test Chemical	6-Methoxy-2-benzothiazolamine	2.5	91	84	181	66	82*
			95	127	263	92
			84	95	221	88
		5	109	54	285	87	124*
			63	30	285	151
			114	28	455	134
		7.5	49	14	266	180	180*
			57	27	283	166
			48	21	278	195
		10

		15


Positive Control	3-Methylcholanthrene	2.5	61	45	705	383	368*
			61	26	575	313
			67	50	825	408

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination