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Study Information
Study Attribute Value
CEBS Accession Number: 002-01554-0011-0000-9
Chemical Name: 6-Methoxy-2-benzothiazolamine
CASRN: 1747-60-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 861345
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 105 111 86 27 30
    104 106 85 27
    95 103 84 29
    81 80 86 36
Test Chemical 6-Methoxy-2-benzothiazolamine 
100         
 105 82 101 32 33
    103 92 108 35
    111 75 102 31
  
200         
 79 50 106 45 38
    89 64 92 34
    104 66 106 34
  
300         
 93 54 87 31 29
    90 47 78 29
    100 50 77 26
  
400         
 90 34 190 71 59*
    121r 32 268 74
    120 46 168 47
  
600         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 58 55 425 245 266*
    54 43 450 277
    57 48 472 275

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 119# 99 91 26 27
    97# 102 71 24
    110 107 114 35
    106# 92 77 24
Test Chemical 6-Methoxy-2-benzothiazolamine 
100         
 102 79 103 34 32
    117 89 97 28
    120 75 121 34
  
200         
 103 82 101 33 32
    117 75 119 34
    109 68 97 30
  
300         
 112 40 100 30 36
    110 33 131 40
    86 16 102 40
  
400         
 112# 23 155 46 44*
    96# 33 122 42
    121r 36 81 22
  
600         
 110 46 60 18
    122r 34 136 37
  
800         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 81 56 973 399 384*
    87 59 919 353
    71 46 854 401

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 104 103 125 40 40
    124r 82 154 42
    110 94 148 45
    95 102 103 36
Test Chemical 6-Methoxy-2-benzothiazolamine 
50         
 95 62 101 35 42
    101 81 106 35
    83 56 141 57
  
75         
 110 46 121 37 38
    114 51 173 51
    106 40 89 28
  
100         
 87 44 120 46 53
    122r 60 161 44
    106 44 189 59
  
150         
 118 35 188 53 53
    88 17 141 53
    93r 10 211 76
  
200         
   
  
300         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 86 42 1146 447 408*
    85 50 1092 429
    66 42 684 347

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 98 109 35 34
    96 113 123 43
    101 95 78 26
    92 94 86 31
Test Chemical 6-Methoxy-2-benzothiazolamine 
 0.625     
 120 70 143 40 44
    117 92 177 50
    95 80 119 42
  
1.25      
 110 81 185 56 53*
    117 75 162 46
    106 89 178 56
  
2.5       
 126r 87 215 57 87*
    112 59 302 90
    116# 58 293 85
  
5         
 95 21 300 105 189*
    63 9 531 281
    73 12 400 183
  
7.5       
 70 8 409 194 225*
    76 8 456 200
    68 6 573 281
  
10         
 65 5 574 297 341*
    48 3 437 301
    38 3 480 425
  
15         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 101 74 608 201 218*
    96 67 509 177
    92 65 755 275

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 101 105 105 35 28
    105# 88 88 28
    101 95 81 27
    92 113 67 24
Test Chemical 6-Methoxy-2-benzothiazolamine 
2.5       
 91 84 181 66 82*
    95 127 263 92
    84 95 221 88
  
5         
 109 54 285 87 124*
    63 30 285 151
    114 28 455 134
  
7.5       
 49 14 266 180 180*
    57 27 283 166
    48 21 278 195
  
10         
   
  
15         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 61 45 705 383 368*
    61 26 575 313
    67 50 825 408

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination