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Study Information
Study Attribute Value
CEBS Accession Number: 002-01557-0001-0000-1
Chemical Name: 6-Nitrobenzimidazole
CASRN: 94-52-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 753791_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 05/05/1987    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 435 0.415 8.70 25.8 0.000
Test Chemical 6-Nitrobenzimidazole 
2.3       
 50 1040 453 0.436 9.06 25.8 4.939 
7.5       
 50 1050 463 0.441 9.26 25.8 6.234 
22.5       
 50 1047 486 0.464 9.72 25.8 11.831 
75         
 0 0 0 0.000 0.00 36.3 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1035 632 0.611 12.64 25.8 47.112
Mitomycin-C 
 0.01      
 5 104 236 2.269 47.20 25.8 446.702
Trend: 1.664        
Probability: 0.048        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/05/1987    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 441 0.421 8.82 25.8 0.000
Test Chemical 6-Nitrobenzimidazole 
225         
 50 1042 448 0.430 8.96 25.8 2.172 
750         
 50 1043 429 0.411 8.58 25.8 -2.255 
2250         
 50 1044 536 0.513 10.72 34.0 22.008
Positive Control Cyclophosphamide 
 0.4       
 50 1048 560 0.534 11.20 25.8 26.984
Cyclophosphamide 
2         
 5 105 158 1.505 31.60 25.8 257.594
Trend: 2.766        
Probability: 0.003        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 06/08/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 524 159 0.303 6.36 25.7 0.000
Test Chemical 6-Nitrobenzimidazole 
1242         
 25 525 216 0.411 8.64 25.7 35.590 
1496         
 25 523 185 0.354 7.40 25.7 16.575 
1760         
 25 523 226 0.432 9.04 30.7 42.410 
2003         
 25 522 271 0.519 10.84 30.7 71.093 
2250         
 25 523 188 0.359 7.52 25.7 18.465 
2500         
 25 521 185 0.355 7.40 25.7 17.022
Positive Control Cyclophosphamide 
 0.4       
 25 525 396 0.754 15.84 25.7 148.582
Cyclophosphamide 
2         
 5 104 148 1.423 29.60 25.7 368.989
Trend: 1.827        
Probability: 0.034