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Study Information
Study Attribute Value
CEBS Accession Number: 002-01557-0002-0000-2
Chemical Name: 6-Nitrobenzimidazole
CASRN: 94-52-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 753791_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #:1   Activation: No Activation    Date: 07/08/1987    Harvest Time: 20.2 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 6-Nitrobenzimidazole 
500         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
650         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
750         
 100 8 0.080 8.0 0 0.000 0.0 8 0.080 8.0 0 0.000 0.0 
850         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 50 31 0.620 36.0 13 0.260 20.0 18 0.360 28.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 14 0.560 44.0 4 0.160 16.0 10 0.400 36.0 0 0.000 0.0
Trend: 1.661 -0.727 2.142  
Probability: 0.048 0.766 0.016  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/22/1989    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 6-Nitrobenzimidazole 
1250         
 25 26 1.040 60.0 8 0.320 24.0 18 0.720 60.0 0 0.000 0.0 
1875         
 25 40 1.600 84.0 8 0.320 24.0 32 1.280 80.0 0 0.000 0.0 
2500         
 25 61 2.440 100.0 15 0.600 36.0 46 1.840 100.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 23 0.230 19.0 5 0.050 5.0 18 0.180 14.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 28 1.120 60.0 11 0.440 36.0 17 0.680 44.0 0 0.000 0.0
Trend: 10.953 5.561 10.815  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 06/23/1987    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Test Chemical: 6-Nitrobenzimidazole 
250         
 200 16 0.080 6.0 0 0.000 0.0 16 0.080 6.0 0 0.000 0.0 
500         
 200 11 0.055 5.0 1 0.005 0.5 10 0.050 4.5 0 0.000 0.0 
750         
 100 25 0.250 15.0 0 0.000 0.0 15 0.150 14.0 10 0.100 1.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 50 26 0.520 36.0 14 0.280 22.0 12 0.240 18.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 100 10 0.100 10.0 1 0.010 1.0 9 0.090 9.0 0 0.000 0.0
Trend: 3.767 -0.269 3.711  
Probability: 0.000 0.606 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 06/23/1987    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: 6-Nitrobenzimidazole 
1250         
 25 22 0.880 68.0 3 0.120 12.0 19 0.760 64.0 0 0.000 0.0 
1875         
 10 15 1.500 100.0 1 0.100 10.0 14 1.400 100.0 0 0.000 0.0 
2500         
 25 21 0.840 76.0 0 0.000 0.0 21 0.840 76.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 21 0.105 9.5 5 0.025 2.0 16 0.080 7.5 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 13 0.520 44.0 2 0.080 8.0 11 0.440 40.0 0 0.000 0.0
Trend: 11.838 1.015 12.191  
Probability: 0.000 0.155 0.000